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Ameliorated healing of biliary anastomosis by autologous adipose-derived stem cell sheets
http://hdl.handle.net/10069/39634
http://hdl.handle.net/10069/3963427b97aa1-d813-433c-8b8c-61a511ba4a24
名前 / ファイル | ライセンス | アクション |
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RegThe14_79.pdf (2.2 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2020-01-28 | |||||
タイトル | ||||||
タイトル | Ameliorated healing of biliary anastomosis by autologous adipose-derived stem cell sheets | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Adipose-derived stem cell | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Cell sheet | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Biliary anastomosis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Anastomotic healing | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Hara, Takanobu
× Hara, Takanobu× Soyama, Akihiko× Adachi, Toshiyuki× Kobayashi, Shinichiro× Sakai, Yusuke× Maruya, Yasuhiro× Kugiyama, Tota× Hidaka, Masaaki× Okada, Satomi× Hamada, Takashi× Maekawa, Kyoichiro× Ono, Shinichiro× Adachi, Tomohiko× Takatsuki, Mitsuhisa× Eguchi, Susumu |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Introduction: Cell sheets consisting of adipose-derived stem cells (ADSCs) have been reported to be effective for wound healing. We conducted this study to clarify the efficacy of ADSC sheets in wound healing at the duct-to-duct biliary anastomotic site in pigs. Methods: Eleven female pigs (20?25 kg) were divided into two groups: biliary anastomosis with an ADSC sheet (n = 6) or without an ADSC sheet (n = 5). To follow the transplanted ADSCs, PKH26GL-labeled sheets were used in one of the ADSC pigs. Two weeks prior to laparotomy, ADSCs were isolated from the lower abdominal subcutaneous adipose tissue. After three passages, ADSCs were seeded on temperature-responsive culture dishes and collected as cell sheets. ADSC sheets were gently transplanted on the anastomotic site. We evaluated specimens by PKH26GL labeling, macroscopic changes, infiltration of inflammatory cells, and collagen content. Results: Labeled ADSCs remained around the bile duct wall. In the no-ADSC group, more adhesion developed at the hepatic hilum as observed during relaparotomy. Histopathological examination showed that the diameter and cross-sectional area of the bile duct wall were decreased in the ADSC group. In the no-ADSC group, a large number of inflammatory cells and more collagen fibers were identified in the bile duct wall. Conclusions: The present study demonstrated that autologous ADSC sheet transplantation reduced hypertrophic changes in the bile duct wall at the anastomotic site. A long-term follow-up is required to evaluate the efficacy of this mechanism in prevention of biliary anastomotic strictures. | |||||
書誌情報 |
Regenerative Therapy 巻 14, p. 79-86, 発行日 2020-01-17 |
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出版者 | ||||||
出版者 | 日本再生医療学会 | |||||
出版者別言語 | ||||||
The Japanese Society for Regenerative Medicine | ||||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 23523204 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.reth.2019.11.001 | |||||
権利 | ||||||
権利情報 | c 2020, The Japanese Society for Regenerative Medicine. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Regenerative Therapy, 14, pp.79-86; 2020 |