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The aim of this study is to investigate a novel diagnostic method, targeting genomic instability (GIN) in intracystic tumors of the breast, using tumor DNA from samples obtained by fine-needle aspiration biopsy (FNAB). Methods: Thirteen consecutive intracystic tumors of the breast, including five cancers and eight benign tumors, were studied. Three FNAB passages per tumor were used for array comparative genomic hybridization (aCGH) analysis to quantify GIN in each tumor. Tumor DNA from the main tumor, taken from formalin-fixed, paraffin-embedded (FFPE) blocks corresponding to FNAB samples, was also analyzed to compare cytogenetic profiles between these sample types. Results: After three FNAB passages, an average of 7.09 μg (0.24?25.0 μg) of DNA was obtained. The quality of the DNA and the aCGH data was excellent, as judged by the mean derivative log ratio spread (DLRSpread) of 0.22 (0.15?0.29). 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A novel diagnostic method targeting genomic instability in intracystic tumors of the breast
http://hdl.handle.net/10069/35878
http://hdl.handle.net/10069/358784833ed46-ebab-4769-85d7-a27693753250
名前 / ファイル | ライセンス | アクション |
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BreCan22_529.pdf (640.7 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2016-10-01 | |||||
タイトル | ||||||
タイトル | A novel diagnostic method targeting genomic instability in intracystic tumors of the breast | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Array CGH | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Breast cancer | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Diagnostic | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Genomic instability | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Intracystic tumor | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Oikawa, Masahiro
× Oikawa, Masahiro× Yano, Hiroshi× Matsumoto, Megumi× Otsubo, Ryota× Shibata, Kenichiro× Hayashi, Tomayoshi× Abe, Kuniko× Kinoshita, Naoe× Yoshiura, Koh-ichiro× Nagayasu, Takeshi |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Background: Even after needle biopsy, the preoperative differential diagnoses of intracystic tumors of the breast are challenging because of their nonspecific radiological characteristics and subtle cytological and histological appearance. The aim of this study is to investigate a novel diagnostic method, targeting genomic instability (GIN) in intracystic tumors of the breast, using tumor DNA from samples obtained by fine-needle aspiration biopsy (FNAB). Methods: Thirteen consecutive intracystic tumors of the breast, including five cancers and eight benign tumors, were studied. Three FNAB passages per tumor were used for array comparative genomic hybridization (aCGH) analysis to quantify GIN in each tumor. Tumor DNA from the main tumor, taken from formalin-fixed, paraffin-embedded (FFPE) blocks corresponding to FNAB samples, was also analyzed to compare cytogenetic profiles between these sample types. Results: After three FNAB passages, an average of 7.09 μg (0.24?25.0 μg) of DNA was obtained. The quality of the DNA and the aCGH data was excellent, as judged by the mean derivative log ratio spread (DLRSpread) of 0.22 (0.15?0.29). The cytogenetic profiles of paired FNAB and main tumor FFPE samples were highly similar, with an average concordance rate of 97.7 % (81.2?100 %). aCGH analysis from FNAB samples showed significantly more GIN in cancers than in benign tumors, with mean frequencies of aberrant chromosomal regions of 17.5 and 0.34 %, respectively (Wilcoxon’s rank sum test, P = 0.0016). Conclusions: Our novel diagnostic method, which targets GIN, can clearly distinguish cancers from benign tumors of breast intracystic lesions with minimal invasion, thereby avoiding the need for surgical excisional biopsy. | |||||
書誌情報 |
Breast Cancer 巻 22, 号 5, p. 529-535, 発行日 2015-09 |
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出版者 | ||||||
出版者 | 日本乳癌学会 | |||||
出版者別言語 | ||||||
Japanese Breast Cancer Society | ||||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13406868 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 18804233 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1007/s12282-013-0516-9 | |||||
権利 | ||||||
権利情報 | c 2014, The Japanese Breast Cancer Society. | |||||
権利 | ||||||
権利情報 | The final publication is available at link.springer.com | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Breast Cancer, 22(5), pp.529-535; 2015 |