WEKO3
アイテム
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A novel Plasmodium yoelii pseudokinase, PypPK1, is involved in erythrocyte invasion and exflagellation center formation
http://hdl.handle.net/10069/39655
http://hdl.handle.net/10069/396558b30bf37-fb1f-4680-81dd-5f604427689b
名前 / ファイル | ライセンス | アクション |
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ParInt76_102056.pdf (3.2 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2020-02-05 | |||||
タイトル | ||||||
タイトル | A novel Plasmodium yoelii pseudokinase, PypPK1, is involved in erythrocyte invasion and exflagellation center formation | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Plasmodium yoelii | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Pseudokinase | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Exflagellation center | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Sexual stage | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Erythrocyte invasion | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Malaria | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Ishizaki, Takahiro
× Ishizaki, Takahiro× Chaiyawong, Nattawat× Hakimi, Hassan× Asada, Masahito× Tachibana, Mayumi× Ishino, Tomoko× Yahata, Kazuhide× Kaneko, Osamu |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Malaria parasites proliferate by repeated invasion of and multiplication within erythrocytes in the vertebrate host. Sexually committed intraerythrocytic parasites undergo sexual stage differentiation to become gametocytes. After ingestion by the mosquito, male and female gametocytes egress from erythrocytes and fertilize within the mosquito midgut. A complex signaling pathway likely responds to environmental events to trigger gametogenesis and regulate fertilization; however, such knowledge remains limited for malaria parasites. Several pseudokinases are highly transcribed at the gametocyte stage and are possible multi-functional regulators controlling critical steps of the life cycle. Here we characterized one pseudokinase, termed PypPK1, in Plasmodium yoelii that is highly expressed in schizonts and male gametocytes. Immunofluorescence assays for parasites expressing Myc-tagged PypPK1 confirmed that PypPK1 protein is expressed in schizonts and sexual stage parasites. Transgenic ΔpPK1 parasites, in which the PypPK1 gene locus was deleted by the CRISPR/Cas9 method, showed significant growth defect and reduced virulence in mice. In the blood stage, ΔpPK1 parasites were able to egress from erythrocytes similar to wild type parasites; however, erythrocyte invasion efficacy was significantly reduced. During sexual stage development, no clear changes were seen in male and female gametocytemias as well as gametocyte egress from erythrocytes; but, the number of exflagellation centers and oocysts were significantly reduced in ΔpPK1 parasites. Taken together, PypPK1 has an important role for both erythrocyte invasion and exflagellation center formation. | |||||
書誌情報 |
Parasitology International 巻 76, p. 102056, 発行日 2020-01-14 |
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出版者 | ||||||
出版者 | Elsevier B.V. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13835769 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.parint.2020.102056 | |||||
権利 | ||||||
権利情報 | c 2020 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY license(http://creativecommons.org/licenses/BY/4.0/). | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Parasitology International, 76, art.no.102056; 2020 |