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Intratracheal Administration of Recombinant Human Keratinocyte Growth Factor Promotes Alveolar Epithelial Cell Proliferation during Compensatory Lung Growth in Rat
http://hdl.handle.net/10069/34164
http://hdl.handle.net/10069/341647258b1ad-f141-4bfb-b247-6990f0712a56
名前 / ファイル | ライセンス | アクション |
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AHC46_179.pdf (4.4 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2014-02-23 | |||||
タイトル | ||||||
タイトル | Intratracheal Administration of Recombinant Human Keratinocyte Growth Factor Promotes Alveolar Epithelial Cell Proliferation during Compensatory Lung Growth in Rat | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Compensatory growth | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Intratracheal injection | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | PCNA | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Recombinant human KGF | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Trilobectomy | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Furukawa, Katsuro
× Furukawa, Katsuro× Matsumoto, Keitaro× Nagayasu, Takeshi× Yamamoto-Fukuda, Tomomi× Tobinaga, Shuichi× Abo, Takafumi× Yamasaki, Naoya× Tsuchiya, Tomoshi× Miyazaki, Takuro× Kamohara, Ryotaro× Nanashima, Atsushi× Obatake, Masayuki× Koji, Takehiko |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Keratinocyte growth factor (KGF) is considered to be one of the most important mitogens for lung epithelial cells. The objectives of this study were to confirm the effectiveness of intratracheal injection of recombinant human KGF (rhKGF) during compensatory lung growth and to optimize the instillation protocol. Here, trilobectomy in adult rat was performed, followed by intratracheal rhKGF instillation with low (0.4 mg/kg) and high (4 mg/kg) doses at various time-points. The proliferation of alveolar cells was assessed by the immunostaining for proliferating cell nuclear antigen (PCNA) in the residual lung. We also investigated other immunohistochemical parameters such as KGF, KGF receptor and surfactant protein A as well as terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling. Consequently, intratracheal single injection of rhKGF in high dose group significantly increased PCNA labeling index (LI) of alveolar cells in the remaining lung. Surprisingly, there was no difference in PCNA LI between low and high doses of rhKGF with daily injection, and PCNA LI reached a plateau level with 2 days-consecutive administration (about 60%). Our results indicate that even at low dose, daily intratracheal injection is effective to maintain high proliferative states during the early phase of compensatory lung growth. | |||||
書誌情報 |
ACTA HISTOCHEMICA ET CYTOCHEMICA 巻 46, 号 6, p. 179-185, 発行日 2013-12-28 |
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出版者 | ||||||
出版者 | 日本組織細胞化学会 | |||||
出版者別言語 | ||||||
Japan Society of Histochemistry and Cytochemistry | ||||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00445991 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13475800 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1267/ahc.13036 | |||||
権利 | ||||||
権利情報 | c 2013 The Japan Society of Histochemistry and Cytochemistry | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | ACTA HISTOCHEMICA ET CYTOCHEMICA, 46(6), pp.179-185; 2013 |