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Protein Transfection Study Using Multicellular Tumor Spheroids of Human Hepatoma Huh-7 Cells
http://hdl.handle.net/10069/34180
http://hdl.handle.net/10069/341805c37f860-17f1-415b-8d81-1d7993c97c87
名前 / ファイル | ライセンス | アクション |
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PLoS8_82876.pdf (752.5 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2014-03-05 | |||||
タイトル | ||||||
タイトル | Protein Transfection Study Using Multicellular Tumor Spheroids of Human Hepatoma Huh-7 Cells | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Kato, Takuma
× Kato, Takuma× Tanaka, Masakazu× Oba, Makoto |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Several protein transfection reagents are commercially available and are powerful tools for elucidating function of a protein in a cell. Here we described protein transfection studies of the commercially available reagents, Pro- DeliverIN, Xfect, and TuboFect, using Huh-7 multicellular tumor spheroid (MCTS) as a three-dimensional in vitro tumor model. A cellular uptake study using specific endocytosis inhibitors revealed that each reagent was internalized into Huh-7 MCTS by different mechanisms, which were the same as monolayer cultured Huh-7 cells. A certain amount of Pro-DeliverIN and Xfect was uptaken by Huh-7 cells through caveolae-mediated endocytosis, which may lead to transcytosis through the surface-first layered cells of MCTS. The results presented here will help in the choice and use of protein transfection reagents for evaluating anti-tumor therapeutic proteins against MCTS models. | |||||
書誌情報 |
PLoS ONE 巻 8, 号 12, p. e82876, 発行日 2013-12-05 |
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出版者 | ||||||
出版者 | Public Library of Science | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 19326203 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1371/journal.pone.0082876 | |||||
権利 | ||||||
権利情報 | c 2013 Kato et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | PLoS ONE, 8(12), e82876; 2013 |