WEKO3
アイテム
{"_buckets": {"deposit": "16b105ae-b2af-4703-a279-22815970d3cf"}, "_deposit": {"created_by": 2, "id": "5286", "owners": [2], "pid": {"revision_id": 0, "type": "depid", "value": "5286"}, "status": "published"}, "_oai": {"id": "oai:nagasaki-u.repo.nii.ac.jp:00005286", "sets": ["28"]}, "author_link": ["22174", "22168", "22170", "22172", "22171", "22173", "22169", "22175"], "item_2_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2014-02-11", "bibliographicIssueDateType": "Issued"}, "bibliographicPageEnd": "4", "bibliographicPageStart": "1", "bibliographicVolumeNumber": "2014", "bibliographic_titles": [{"bibliographic_title": "BioMed Research International"}]}]}, "item_2_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "D-Glucosamine is a useful medicament in various fields of medicine and dentistry. With respect to stability of the cell membrane, it has been reported that bradykinin-induced nociceptive responses are significantly suppressed by the direct application of D-glucosamine. Electroporation is usually used to effectively introduce foreign genes into tissue culture cells. Buffers for electroporation with or without D-glucosamine are used in experiments of transfection vectors. This is the first study to indirectly observe the stability and protection of the osteoblast membrane against both electric stress and gene uptake (the proton sponge hypothesis: osmotic rupture during endosomes prior to fusion with lysosomes) in electroporation with D-glucosamine application. The transfection efficiency was evaluated as the fluorescence intensity of the transfected green fluorescent protein (GFP) in the cultured cells (osteoblasts; NOS-1 cells). The transfection efficiency increased over 30% in the electroporation samples treated with D-glucosamine-supplemented buffer after one day. The membrane absorption of D-glucosamine is the primary mechanism of membrane stress induced by electric stress. This new function of D-glucosamine is useful and meaningful for developing more effective transformation procedures.", "subitem_description_type": "Abstract"}]}, "item_2_description_63": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "BioMed Research International, 2014, 485867; 2014", "subitem_description_type": "Other"}]}, "item_2_publisher_33": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Hindawi Publishing Corporation"}]}, "item_2_relation_12": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1155/2014/485867", "subitem_relation_type_select": "DOI"}}]}, "item_2_rights_13": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "c 2014 Kazunari Igawa et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited."}]}, "item_2_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "23146133", "subitem_source_identifier_type": "ISSN"}]}, "item_2_source_id_8": {"attribute_name": "EISSN", "attribute_value_mlt": [{"subitem_source_identifier": "23146141", "subitem_source_identifier_type": "ISSN"}]}, "item_2_version_type_16": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Igawa, Kazunari"}], "nameIdentifiers": [{"nameIdentifier": "22168", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ohara, Naoko"}], "nameIdentifiers": [{"nameIdentifier": "22169", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kawakubo, Atsushi"}], "nameIdentifiers": [{"nameIdentifier": "22170", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sugimoto, Kouji"}], "nameIdentifiers": [{"nameIdentifier": "22171", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yanagiguchi, Kajiro"}], "nameIdentifiers": [{"nameIdentifier": "22172", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ikeda, Takeshi"}], "nameIdentifiers": [{"nameIdentifier": "22173", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yamada, Shizuka"}], "nameIdentifiers": [{"nameIdentifier": "22174", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hayashi, Yoshihiko"}], "nameIdentifiers": [{"nameIdentifier": "22175", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-12-21"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "BMRI2014_485867.pdf", "filesize": [{"value": "1.7 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 1700000.0, "url": {"label": "BMRI2014_485867.pdf", "url": "https://nagasaki-u.repo.nii.ac.jp/record/5286/files/BMRI2014_485867.pdf"}, "version_id": "7a87c67d-c3ee-4a9b-af50-04b352e858cc"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "D-Glucosamine Promotes Transfection Efficiency during Electroporation", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "D-Glucosamine Promotes Transfection Efficiency during Electroporation"}]}, "item_type_id": "2", "owner": "2", "path": ["28"], "permalink_uri": "http://hdl.handle.net/10069/34346", "pubdate": {"attribute_name": "公開日", "attribute_value": "2014-05-07"}, "publish_date": "2014-05-07", "publish_status": "0", "recid": "5286", "relation": {}, "relation_version_is_last": true, "title": ["D-Glucosamine Promotes Transfection Efficiency during Electroporation"], "weko_shared_id": -1}
D-Glucosamine Promotes Transfection Efficiency during Electroporation
http://hdl.handle.net/10069/34346
http://hdl.handle.net/10069/34346274fb797-e532-498a-90a3-f0a81574b25b
名前 / ファイル | ライセンス | アクション |
---|---|---|
BMRI2014_485867.pdf (1.7 MB)
|
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2014-05-07 | |||||
タイトル | ||||||
タイトル | D-Glucosamine Promotes Transfection Efficiency during Electroporation | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Igawa, Kazunari
× Igawa, Kazunari× Ohara, Naoko× Kawakubo, Atsushi× Sugimoto, Kouji× Yanagiguchi, Kajiro× Ikeda, Takeshi× Yamada, Shizuka× Hayashi, Yoshihiko |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | D-Glucosamine is a useful medicament in various fields of medicine and dentistry. With respect to stability of the cell membrane, it has been reported that bradykinin-induced nociceptive responses are significantly suppressed by the direct application of D-glucosamine. Electroporation is usually used to effectively introduce foreign genes into tissue culture cells. Buffers for electroporation with or without D-glucosamine are used in experiments of transfection vectors. This is the first study to indirectly observe the stability and protection of the osteoblast membrane against both electric stress and gene uptake (the proton sponge hypothesis: osmotic rupture during endosomes prior to fusion with lysosomes) in electroporation with D-glucosamine application. The transfection efficiency was evaluated as the fluorescence intensity of the transfected green fluorescent protein (GFP) in the cultured cells (osteoblasts; NOS-1 cells). The transfection efficiency increased over 30% in the electroporation samples treated with D-glucosamine-supplemented buffer after one day. The membrane absorption of D-glucosamine is the primary mechanism of membrane stress induced by electric stress. This new function of D-glucosamine is useful and meaningful for developing more effective transformation procedures. | |||||
書誌情報 |
BioMed Research International 巻 2014, p. 1-4, 発行日 2014-02-11 |
|||||
出版者 | ||||||
出版者 | Hindawi Publishing Corporation | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 23146133 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 23146141 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1155/2014/485867 | |||||
権利 | ||||||
権利情報 | c 2014 Kazunari Igawa et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | BioMed Research International, 2014, 485867; 2014 |