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Hepatocyte growth factor enhances the barrier function in primary cultures of rat brain microvascular endothelial cells
http://hdl.handle.net/10069/34604
http://hdl.handle.net/10069/34604051981b2-f519-45b2-bbbc-8227762f7344
名前 / ファイル | ライセンス | アクション |
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MicRes92_4.pdf (1.9 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2014-07-31 | |||||
タイトル | ||||||
タイトル | Hepatocyte growth factor enhances the barrier function in primary cultures of rat brain microvascular endothelial cells | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Hepatocyte growth factor | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Blood-brain barrier | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Tight junctions | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Adherens junction | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | F-actin, ZO-1 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | VE-cadherin | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Peripheral endothelial cells | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | TEER | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | permeability | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Yamada, Narumi
× Yamada, Narumi× Nakagawa, Shinsuke× Horai, Shoji× Tanaka, Kunihiko× Deli, Maria A.× Yatsuhashi, Hiroshi× Niwa, Masami |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The effects of hepatocyte growth factor (HGF) on barrier functions were investigated by a blood-brain barrier (BBB) in vitro model comprising a primary culture of rat brain capillary endothelial cells (RBEC). In order to examine the response of the peripheral endothelial cells to HGF, human umbilical vascular endothelial cells (HUVEC) and human dermal microvascular endothelial cells (HMVEC) were also treated with HGF. HGF decreased the permeability of RBEC to sodium fluorescein and Evans blue albumin, and dose-dependently increased transendothelial electrical resistance (TEER) in RBEC. HGF altered the immunochemical staining pattern of F-actin bands and made ZO-1 staining more distinct on the linear cell borders in RBEC. In contrast, HGF increased sodium fluorescein and Evans blue albumin permeability in HMVEC and HUVEC, and decreased TEER in HMVEC. In HMVEC, HGF reduced cortical actin bands and increased stress fiber density, and increased the zipper-like appearance of ZO-1 staining. Western blot analysis showed that HGF significantly increased the amount of ZO-1 and VE-cadherin. HGF seems to act on the BBB to strengthen BBB integrity. These findings indicated that cytoskeletal rearrangement and cell-cell adhesion, such as through VE-cadherin and ZO-1, are candidate mechanisms for the influence of HGF on the BBB. The possibility that HGF has therapeutic significance in protecting the BBB from damage needs to be considered. | |||||
書誌情報 |
Microvascular Research 巻 92, p. 41-49, 発行日 2014-03 |
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出版者 | ||||||
出版者 | Academic Press Inc. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00262862 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.mvr.2013.12.004 | |||||
権利 | ||||||
権利情報 | c 2013 Elsevier Inc. | |||||
権利 | ||||||
権利情報 | NOTICE: this is the author’s version of a work that was accepted for publication in Microvascular Research. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Microvascular Research, 92, (2014) | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Microvascular Research, 92, pp.41-49; 2014 |