WEKO3
アイテム
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Persistent prion infection disturbs the function of Oct-1, resulting in the down-regulation of murine interferon regulatory factor-3
http://hdl.handle.net/10069/34768
http://hdl.handle.net/10069/34768ba2d0384-8100-41b1-93e6-c993577dc11f
名前 / ファイル | ライセンス | アクション |
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SciRep4_6006.pdf (682.9 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2014-10-10 | |||||
タイトル | ||||||
タイトル | Persistent prion infection disturbs the function of Oct-1, resulting in the down-regulation of murine interferon regulatory factor-3 | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Homma, Takujiro
× Homma, Takujiro× Ishibashi, Daisuke× Nakagaki, Takehiro× Fuse, Takayuki× Sano, Kazunori× Satoh, Katsuya× Atarashi, Ryuichiro× Nishida, Noriyuki |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | As a prompt response against invasion of various viruses, interferon regulatory factor-3 (IRF-3) is initially phosphorylated to become activated and upregulates mainly Type I Interferons (IFN-I) in most cell types. We previously reported that IRF-3-dependent host innate immune responses partially interfere in infection of prions. Here, we found that stable infection of prion suppressed IRF-3 gene-expression. The decreased promoter activity of IRF-3 was significantly restored along with treatment of anti-prion drugs in the prion-infected cells, suggesting that infection of prion directly influence the regulation of IRF-3 transcription. We further investigated promoter activity of 5'- flanking region of murine IRF-3 using a luciferase reporter system and found that the nucleotides -119 to -1 were indispensable for the promoter activity. Within this region, mutations in the Oct-1 binding site significantly reduced the promoter activity and chromatin immunoprecipitation (ChIP) assay revealed that Oct-1 indeed binds to the region. In addition, overexpression of Oct-1 increased the promoter activity of IRF-3. Intriguingly, Oct-1 protein was significantly reduced in prion-infected cells and mice brains compared with uninfected groups. Taken together, we concluded that prion infection could interfere in the function of Oct-1, resulting in the down-regulation of IRF-3. | |||||
書誌情報 |
Scientific Reports 巻 4, p. 6006, 発行日 2014-08-08 |
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出版者 | ||||||
出版者 | Nature Publishing Group | |||||
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収録物識別子タイプ | ISSN | |||||
収録物識別子 | 20452322 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1038/srep06006 | |||||
権利 | ||||||
権利情報 | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/ | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Scientific Reports, 4, 6006; 2014 |