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Biodegradable nanoparticles composed of dendrigraft poly-l-lysine for gene delivery
http://hdl.handle.net/10069/34974
http://hdl.handle.net/10069/3497458d2cd7d-b8c5-4d63-8ed3-f7c084b64693
名前 / ファイル | ライセンス | アクション |
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EJPB87_472.pdf (415.5 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2014-12-26 | |||||
タイトル | ||||||
タイトル | Biodegradable nanoparticles composed of dendrigraft poly-l-lysine for gene delivery | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Biodegradable | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Dendrigraft poly-l-lysine | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Gene delivery | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Nanoparticle | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Ternary complex | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | γ-Polyglutamic acid | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Kodama, Yukinobu
× Kodama, Yukinobu× Nakamura, Tadahiro× Kurosaki, Tomoaki× Egashira, Kanoko× Mine, Toyoharu× Nakagawa, Hiroo× Muro, Takahiro× Kitahara, Takashi× Higuchi, Norihide× Sasaki, Hitoshi |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | We developed novel gene vectors composed of dendrigraft poly-l-lysine (DGL). The transgene expression efficiency of the pDNA/DGL complexes (DGL complexes) was markedly higher than that of the control pDNA/poly-l-lysine complex. However, the DGL complexes caused cytotoxicity and erythrocyte agglutination at high doses. Therefore, γ-polyglutamic acid (γ-PGA), which is a biodegradable anionic polymer, was added to the DGL complexes to decrease their toxicity. The resultant ternary complexes (DGL/γ-PGA complexes) were shown to be stable nanoparticles, and those with γ-PGA to pDNA charge ratios of >8 had anionic surface charges. The transgene expression efficiency of the DGL/γ-PGA complexes was similar to that of the DGL complexes; however, they exhibited lower cytotoxicity and did not induce erythrocyte agglutination at high doses. After being intravenously administered to mice, the DGL6 complex demonstrated high transfection efficiency in the liver, lungs, and spleen, whereas the DGL6/γ-PGA8 complex only displayed high transfection efficiency in the spleen. Future studies should examine the utility of DGL and DGL/γ-PGA complexes for clinical gene therapy. | |||||
書誌情報 |
European Journal of Pharmaceutics and Biopharmaceutics 巻 87, 号 3, p. 472-479, 発行日 2014-08 |
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出版者 | ||||||
出版者 | Elsevier | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 09396411 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.ejpb.2014.04.013 | |||||
権利 | ||||||
権利情報 | c 2014 Elsevier B.V. | |||||
権利 | ||||||
権利情報 | NOTICE: this is the author’s version of a work that was accepted for publication in European Journal of Pharmaceutics and Biopharmaceutics. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in European Journal of Pharmaceutics and Biopharmaceutics, 87, 3, (2014) | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | European Journal of Pharmaceutics and Biopharmaceutics, 87(3), pp.472-479; 2014 |