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Chromatographic determination of low-molecular mass unsaturated aliphatic aldehydes with peroxyoxalate chemiluminescence detection after fluorescence labeling with 4-(N,N-dimethylaminosulfonyl)-7-hydrazino-2,1,3-benzoxadiazole
http://hdl.handle.net/10069/34986
http://hdl.handle.net/10069/349869d404573-7feb-44d5-9c17-c430e81481d7
名前 / ファイル | ライセンス | アクション |
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JCB953_147.pdf (286.9 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2015-01-08 | |||||
タイトル | ||||||
タイトル | Chromatographic determination of low-molecular mass unsaturated aliphatic aldehydes with peroxyoxalate chemiluminescence detection after fluorescence labeling with 4-(N,N-dimethylaminosulfonyl)-7-hydrazino-2,1,3-benzoxadiazole | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Fluorescence labeling | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Lipid peroxidation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Oxidative stress | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Peroxyoxalate chemiluminescence (PO-CL) | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Serum analysis | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Ali, Marwa Fathy Bakr
× Ali, Marwa Fathy Bakr× Kishikawa, Naoya× Ohyama, Kaname× Mohamed, Horria Abdel-Mageed× Abdel-Wadood, Hanaa Mohamed× Mahmoud, Ashraf Mohamed× Imazato, Takahiro× Ueki, Yukitaka× Wada, Mitsuhiro× Kuroda, Naotaka |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | A highly sensitive, selective and reproducible chromatographic method is described for determination of low-molecular mass unsaturated aliphatic aldehydes in human serum. The method combines fluorescent labeling using 4-(N,N-Dimethylaminosulfonyl)-7-hydrazino-2,1,3-benzoxadiazole with peroxyoxalate chemiluminescence. The derivatives were separated on a reversed-phase column C8 isocratically using a mixture of acetonitrile and 90mM imidazole-HNO3 buffer (pH 6.4, 1:1, % v/v). The calibration ranges were: 20-420nM for methylglyoxal, 16-320nM for acrolein, 15-360nM for crotonaldehyde and 20-320nM for trans-2-hexenal. The detection limits were ranged from 4.4 to 6.5nM (88-130fmol/injection), the recovery results were within the range of 87.4-103.8% and the intra and inter-day precision results were lower than 5.5%. The proposed validated method has been successfully applied to healthy, diabetic and rheumatic arthritis patients' sera with simple pretreatment method. In conclusion, this new method is suitable for routine analysis of large numbers of clinical samples for assessment of the oxidative stress state in patients. | |||||
書誌情報 |
Journal of Chromatography B 巻 953-954, p. 147-152, 発行日 2014-03-15 |
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出版者 | ||||||
出版者 | Elsevier | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 15700232 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.jchromb.2014.02.009 | |||||
権利 | ||||||
権利情報 | c 2014 Published by Elsevier B.V. | |||||
権利 | ||||||
権利情報 | NOTICE: this is the author’s version of a work that was accepted for publication in Journal of Chromatography B. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Chromatography B, 953-954, (2014) | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Journal of Chromatography B, 953-954; pp.147-152; 2014 |