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化学発光法に基づく生体内活性酸素産生物質の解析
http://hdl.handle.net/10069/35114
http://hdl.handle.net/10069/35114af613a1c-eca6-4003-a089-b03dbc05f981
名前 / ファイル | ライセンス | アクション |
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YakZas135_191.pdf (560.4 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2015-03-03 | |||||
タイトル | ||||||
タイトル | 化学発光法に基づく生体内活性酸素産生物質の解析 | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Chemiluminescence | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | High-performance liquid chromatography (HPLC) | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Paraquat | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Quinone | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Reactive oxygen species | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
岸川, 直哉
× 岸川, 直哉× 黒田, 直敬 |
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著者別名 | ||||||
姓名 | Kishikawa, Naoya | |||||
著者別名 | ||||||
姓名 | Kuroda, Naotaka | |||||
その他のタイトル | ||||||
その他のタイトル | Chemiluminescence Assay for the Investigation of Reactive Oxygen Species Generator | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Quinones play critical roles in biological systems, but are also regarded as a class of toxins that can cause oxidative stress in living cells, and the involvement of quinone-based reactive oxygen species in oxidative stress has been reported. In biological systems, quinones are reduced to semiquinone radicals by the enzyme NADPH:quinone reductase. Next, semiquinone radicals react with dissolved oxygen to form superoxide anion, which reacts with biological molecules to cause oxidative stress. On the other hand, chemiluminescence reagents such as luminol can emit chemiluminescence after oxidation by reactive oxygen species. Therefore, chemiluminescence reagents have been used widely to investigate reactive oxygen species. We have developed a sensitive and selective assay for quantifying quinones using luminol chemiluminescence. This chemiluminescence assay is based on the generation of reactive oxygen species through the redox reaction between quinone and dithiothreitol, a reductant, followed by detection of the generated reactive oxygen by luminol. Additionally, this assay can be used to quantify the toxic herbicide, paraquat, which produces reactive oxygen species in the same manner as quinones. This review describes the development of a sensitive and selective chemiluminescence assay for investigating quinones and paraquat by utilizing their ability to generate reactive oxygen species. | |||||
書誌情報 |
YAKUGAKU ZASSHI 巻 135, 号 2, p. 191-196, 発行日 2015-02-01 |
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出版者 | ||||||
出版者 | 日本薬学会 | |||||
出版者別言語 | ||||||
Pharmaceutical Society of Japan | ||||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00316903 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13475231 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1248/yakushi.14-00213-1 | |||||
権利 | ||||||
権利情報 | c 2015 by the PHARMACEUTICAL SOCIETY OF JAPAN | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | YAKUGAKU ZASSHI, 135(2), pp.191-196; 2015 |