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Safe and Effective Delivery of Small Interfering RNA with Polymer- and Liposomes-Based Complexes
http://hdl.handle.net/10069/33126
http://hdl.handle.net/10069/33126b7b3eff0-2b26-43a2-823b-e0e004ef5e03
名前 / ファイル | ライセンス | アクション |
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BPBul36_995.pdf (838.2 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2013-08-05 | |||||
タイトル | ||||||
タイトル | Safe and Effective Delivery of Small Interfering RNA with Polymer- and Liposomes-Based Complexes | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | γ-polyglutamic acid | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Cancer | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Cationic liposome | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Cationic polymer | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Gene delivery | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Small interfering RNA | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Kodama, Yukinobu
× Kodama, Yukinobu× Harauchi, Satoe× Kawanabe, Saki× Ichikawa, Nobuhiro× Nakagawa, Hiroo× Muro, Takahiro× Higuchi, Norihide× Nakamura, Tadahiro× Kitahara, Takashi× Sasaki, Hitoshi |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | We developed binary and ternary complexes based on polymers and liposomes for safe and effective delivery of small interfering RNA (siRNA). Anti-luciferase siRNA was used as a model of nucleic acid medicine. The binary complexes of siRNA were prepared with cationic polymers and cationic liposomes such as polyethylenimine (PEI), polyamidoamine (PAMAM) dendrimer, poly-L-arginine (PLA), trimethyl[2,3-(dioleoxy)-propyl]ammonium chloride (DOTMA), and cholesteryl 3β-N-(dimetylaminnoethyl)carbamate hydrochloride (DC-Chol). The ternary complexes were constructed by the addition of γ-polyglutamic acid (γ-PGA) to the binary complexes. The complexes were approximately 54-153 nm in particle size. The binary complexes showed a cationic surface charge although an anionic surface charge was observed in the ternary complexes. The polymer-based complexes did not show a silencing effect in the mouse colon carcinoma cell line expressing luciferase regularly (Colon26/Luc cells). The binary complexes based on liposomes and their ternary complexes coated by γ-PGA showed a significant silencing effect. The binary complexes showed significant cytotoxicity although the ternary complexes coated by γ-PGA did not show significant cytotoxicity. The ternary complexes coated by γ-PGA suppressed luciferase activity in the tumor after their direct injection into the tumors of mice bearing Colon26/Luc cells. Thus, we have newly identified safe and efficient ternary complexes of siRNA for clinical use. | |||||
書誌情報 |
Biological and Pharmaceutical Bulletin 巻 36, 号 6, p. 995-1001, 発行日 2013-06-01 |
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出版者 | ||||||
出版者 | 日本薬学会 | |||||
出版者別言語 | ||||||
Pharmaceutical Society of Japan | ||||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 09186158 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13475215 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1248/bpb.b13-00054 | |||||
権利 | ||||||
権利情報 | © 2013 The Pharmaceutical Society of Japan | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Biological and Pharmaceutical Bulletin, 36(6), pp.995-1001; 2013 |