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Extracellular vesicles from senescent hepatic stellate cells promote cell viability of hepatoma cells through increasing EGF secretion from differentiated THP?1 cells
http://hdl.handle.net/10069/39793
http://hdl.handle.net/10069/39793e31c017e-504e-4a22-b61d-3c9a8c72a5e1
名前 / ファイル | ライセンス | アクション |
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BioRep12_163.pdf (801.8 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2020-04-09 | |||||
タイトル | ||||||
タイトル | Extracellular vesicles from senescent hepatic stellate cells promote cell viability of hepatoma cells through increasing EGF secretion from differentiated THP?1 cells | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | epidermal growth factor | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | etoposide | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | extracellular vesicles | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | hepatic stellate cells | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | hepatocellular carcinoma | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | senescence?associated secretory phenotype | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Miyazoe, Yuri
× Miyazoe, Yuri× Miuma, Satoshi× Miyaaki, Hisamitsu× Kanda, Yasuko× Nakashiki, Suguru× Sasaki, Ryu× Haraguchi, Masafumi× Shibata, Hidetaka× Honda, Takuya× Taura, Naota× Nakao, Kazuhiko |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Since the discovery of the senescence-associated secretory phenotype, the role of senescent hepatic stellate cells (HSCs) in hepatocellular carcinoma (HCC) development has gained increasing attention. Similar to cytokines, extracellular vesicles (EVs) are essential for intercellular communication. However, the function of EVs derived from senescent HSCs in HCC progression has not been extensively studied. The aims of the present study were to characterize the EVs derived from senescent HSCs and determine their role in the tumor microenvironment. Cellular senescence was induced in human hepatic stellate cells (HHSteCs) with various concentrations of etoposide. Induction was confirmed using EdU staining and 53BP1 and p21 immunostaining. EVs were isolated by ultracentrifugation and analyzed by nanoparticle tracking analysis. Multiplex immunoassays were used to compare the levels of growth factors secreted from hepatoma cell lines and macrophage cells pretreated with EVs derived from senescent HHSteCs (senescent EVs) with those pretreated with EVs derived from normal cultured HHSteCs (normal EVs). Treatment with 25 μM etoposide for 3 days was the most effective at inducing senescence in HHSteCs. This finding was confirmed by induction of irreversible cell-cycle arrest, upregulation of 53BP1 and p21 expression, and increased SA-β-gal staining. Senescent HHSteCs released increased quantities of EV particles compared with normally cultured HHSteCs. Multiplex analysis revealed that there was no difference between hepatoma cell lines treated with normal EVs and those treated with senescent EVs in growth factor secretion. In contrast, the secretion of epidermal growth factor (EGF) was increased by macrophage cells treated with senescent EVs compared with those treated with normal EVs. Furthermore, senescent EVs did not affect the viability of hepatoma cells but increased the viability of hepatoma cells co-cultured with macrophage cells. In conclusion, the release of EVs from senescent HSCs was higher compared with normal HSCs. Furthermore, senescent EVs promoted HCC development by upregulating EGF secretion from macrophages. | |||||
書誌情報 |
Biomedical Reports 巻 12, 号 4, p. 163-170, 発行日 2020-02-14 |
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出版者 | ||||||
出版者 | Spandidos Publications | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 20499434 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 20499442 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.3892/br.2020.1279 | |||||
権利 | ||||||
権利情報 | c Miyazoe et al. This is an open access article distributed under the terms of Creative Commons Attribution License. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Biomedical Reports, 12(4), pp.163-170; 2020 |