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In situ tissue engineering with synthetic self-assembling peptide nanofiber scaffolds, PuraMatrix, for mucosal regeneration in the rat middle-ear
http://hdl.handle.net/10069/33765
http://hdl.handle.net/10069/33765e8f7a1f9-5b6c-461a-b0fc-a57d1fbaeb50
名前 / ファイル | ライセンス | アクション |
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IJN8_2629.pdf (4.7 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2013-09-20 | |||||
タイトル | ||||||
タイトル | In situ tissue engineering with synthetic self-assembling peptide nanofiber scaffolds, PuraMatrix, for mucosal regeneration in the rat middle-ear | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | In situ tissue engineering | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Middle-ear mucosa | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Nanofiber | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Regeneration | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Synthetic self-assembling peptide scaffolds | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Akiyama, Notaro
× Akiyama, Notaro× Yamamoto-Fukuda, Tomomi× Takahashi, Haruo× Koji, Takehiko |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Middle-ear mucosa maintains middle-ear pressure. However, the majority of surgical cases exhibit inadequate middle-ear mucosal regeneration, and mucosal transplantation is necessary in such cases. The aim of the present study was to assess the feasibility of transplantation of isolated mucosal cells encapsulated within synthetic self-assembling peptide nanofiber scaffolds using PuraMatrix, which has been successfully used as scaffolding in tissue engineering, for the repair of damaged middle-ear. Middle-ear bullae with mucosa were removed from Sprague Dawley (SD) transgenic rats, transfected with enhanced green fluorescent protein (EGFP) transgene and excised into small pieces, then cultured up to the third passage. After surgical elimination of middle-ear mucosa in SD recipient rats, donor cells were encapsulated within PuraMatrix and transplanted into these immunosuppressed rats. Primary cultured cells were positive for pancytokeratin but not for vimentin, and retained the character of middle-ear epithelial cells. A high proportion of EGFP-expressing cells were found in the recipient middle-ear after transplantation with PuraMatrix, but not without PuraMatrix. These cells retained normal morphology and function, as confirmed by histological examination, immunohistochemistry, and electron microscopy, and multiplied to form new epithelial and subepithelial layers together with basement membrane. The present study demonstrated the feasibility of transplantation of cultured middle-ear mucosal epithelial cells encapsulated within PuraMatrix for regeneration of surgically eliminated mucosa of the middle-ear in SD rats. | |||||
書誌情報 |
International Journal of Nanomedicine 巻 2013, 号 8, p. 2629-2640, 発行日 2013-07-24 |
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出版者 | ||||||
出版者 | Dove Medical Press Ltd. | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 11782013 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.2147/IJN.S47279 | |||||
権利 | ||||||
権利情報 | © 2013 Akiyama et al, publisher and licensee Dove Medical Press Ltd. This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | International Journal of Nanomedicine, 2013(8), pp.2629-2640; 2013 |