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Initial Contact of Glioblastoma Cells with Existing Normal Brain Endothelial Cells Strengthen the Barrier Function via Fibroblast Growth Factor 2 Secretion: A New In Vitro Blood–Brain Barrier Model
http://hdl.handle.net/10069/33933
http://hdl.handle.net/10069/3393308681bf4-6d68-4592-a106-6c180db20785
名前 / ファイル | ライセンス | アクション |
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CMN33_489.pdf (588.9 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2014-06-01 | |||||
タイトル | ||||||
タイトル | Initial Contact of Glioblastoma Cells with Existing Normal Brain Endothelial Cells Strengthen the Barrier Function via Fibroblast Growth Factor 2 Secretion: A New In Vitro Blood–Brain Barrier Model | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Blood-brain barrier | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Fibroblast growth factor 2 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Glioblastoma | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Rat brain endothelial cells | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Toyoda, Keisuke
× Toyoda, Keisuke× Tanaka, Kunihiko× Nakagawa, Shinsuke× Thuy, Dinh Ha Duy× Ujifuku, Kenta× Kamada, Kensaku× Hayashi, Kentaro× Matsuo, Takayuki× Nagata, Izumi× Niwa, Masami |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Glioblastoma multiforme (GBM) cells invade along the existing normal capillaries in brain. Normal capillary endothelial cells function as the blood-brain barrier (BBB) that limits permeability of chemicals into the brain. To investigate whether GBM cells modulate the BBB function of normal endothelial cells, we developed a new in vitro BBB model with primary cultures of rat brain endothelial cells (RBECs), pericytes, and astrocytes. Cells were plated on a membrane with 8 μm pores, either as a monolayer or as a BBB model with triple layer culture. The BBB model consisted of RBEC on the luminal side as a bottom, and pericytes and astrocytes on the abluminal side as a top of the chamber. Human GBM cell line, LN-18 cells, or lung cancer cell line, NCI-H1299 cells, placed on either the RBEC monolayer or the BBB model increased the transendothelial electrical resistance (TEER) values against the model, which peaked within 72 h after the tumor cell application. The TEER value gradually returned to baseline with LN-18 cells, whereas the value quickly dropped to the baseline in 24 h with NCI-H1299 cells. NCI-H1299 cells invaded into the RBEC layer through the membrane, but LN-18 cells did not. Fibroblast growth factor 2 (FGF-2) strengthens the endothelial cell BBB function by increased occludin and ZO-1 expression. In our model, LN-18 and NCI-H1299 cells secreted FGF-2, and a neutralization antibody to FGF-2 inhibited LN-18 cells enhanced BBB function. These results suggest that FGF-2 would be a novel therapeutic target for GBM in the perivascular invasive front. | |||||
書誌情報 |
Cellular and Molecular Neurobiology 巻 33, 号 4, p. 489-501, 発行日 2013-05 |
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出版者 | ||||||
出版者 | Springer New York | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 02724340 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 15736830 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1007/s10571-013-9913-z | |||||
権利 | ||||||
権利情報 | © 2013 Springer Science+Business Media New York. | |||||
権利 | ||||||
権利情報 | The final publication is available at link.springer.com | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Cellular and Molecular Neurobiology, 33(4), pp.489-501; 2013 |