WEKO3
アイテム
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Quinone-based antibody labeling reagent for enzyme-free chemiluminescent immunoassays. Application to avidin and biotinylated anti-rabbit IgG labeling
http://hdl.handle.net/10069/39950
http://hdl.handle.net/10069/399500fd46655-16cc-4feb-9ea5-57147e5c53eb
名前 / ファイル | ライセンス | アクション |
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Biosens160_112215.pdf (998.7 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2020-12-18 | |||||
タイトル | ||||||
タイトル | Quinone-based antibody labeling reagent for enzyme-free chemiluminescent immunoassays. Application to avidin and biotinylated anti-rabbit IgG labeling | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Non-enzymatic sensor | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Biotin | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Quinone | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Chemiluminescence | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Immunoassay | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Stability | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
El-Maghrabey, Mahmoud
× El-Maghrabey, Mahmoud× Kishikawa, Naoya× Harada, Shiori× Ohyama, Kaname× Kuroda, Naotaka |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Chemiluminescence-enzyme immunoassays make it possible to measure trace components with high sensitivity and selectivity due to the high specificity of the antigen-antibody reaction and the high sensitivity of chemiluminescence assays. However, using an enzyme-labeled antibody suffers from many problems such as low reproducibility due to the instability of the enzyme and inhibition of antigen-antibody reaction due to its steric effect. Therefore, herein we report an innovative non-enzymatic chemiluminescence immunoassays labeling reagent through using quinone as a signal-generating tag coupled with biotin as a binder, to overcome enzymatic labeling problems. Biotinylated-1,4-naphthoquinone (biotin-NQ) was synthesized and characterized and it could produce long-lasting chemiluminescence upon mixing with dithiothreitol and luminol based on the redox cycle of quinone. Biotin-NQ showed exceptional stability towards different stress factors that may be encountered during performing the immunoassay such as high temperatures, highly acidic and alkaline conditions, and repeated freeze-thaw cycles. On the other hand, all these conditions lead to decreased labeling enzyme reactivity due to possible denaturation of its protein structure. Finally, the measurement of the biotin-labeled antibody was successfully performed using biotin-NQ and avidin. As a result, the antibody could be detected down to 25.7 nM which is 2.5 times sensitive than biotin-HRP chemiluminescence-enzyme immunoassays. Moreover, our method was applied successfully for determination of avidin using immobilized biotinylated antibody and biotin-NQ, which simulates immunoassays. Avidin could be detected down to 23.4 nM with excellent linearity (r = 0.996). Accordingly, our developed reagent, biotin-NQ, could be used as a universal highly stable, cost-effective, and steric free non-enzymatic label for immunoassays. | |||||
書誌情報 |
Biosensors and Bioelectronics 巻 160, p. 112215, 発行日 2020-04-18 |
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出版者 | ||||||
出版者 | Elsevier B.V. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 09565663 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.bios.2020.112215 | |||||
権利 | ||||||
権利情報 | c 2020 Elsevier B.V. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Biosensors and Bioelectronics, 160, art.no.112215; 2020 |