WEKO3
アイテム
{"_buckets": {"deposit": "a6314c2c-f6c9-4322-82d2-b17a612f5345"}, "_deposit": {"created_by": 2, "id": "823", "owners": [2], "pid": {"revision_id": 0, "type": "depid", "value": "823"}, "status": "published"}, "_oai": {"id": "oai:nagasaki-u.repo.nii.ac.jp:00000823", "sets": ["36"]}, "author_link": ["3854", "3852", "3853", "3855", "3856"], "item_2_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2020-04-29", "bibliographicIssueDateType": "Issued"}, "bibliographicPageStart": "368", "bibliographicVolumeNumber": "8", "bibliographic_titles": [{"bibliographic_title": "Frontiers in Bioengineering and Biotechnology"}]}]}, "item_2_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Human immunodeficiency virus type 1 (HIV-1)-based viral vector is widely used as a biomaterial to transfer a gene of interest into target cells in many biological study fields including gene therapy. Vesicular stomatitis virus glycoprotein (VSV-G)-containing HIV-1 vector much more efficiently transduces various mammalian cells than other viral envelope proteins-containing vectors. Understanding the mechanism would contribute to development of a novel method of efficient HIV-1 vector production. HIV-1 vector is generally constructed by transient transfection of human 293T or African green monkey COS7 cells. It was found in this study that HIV-1 Gag protein is constitutively digested in lysosomes of African green monkey cells. Surprisingly, VSV-G elevated HIV-1 Gag protein levels, suggesting that VSV-G protects Gag protein from the lysosomal degradation. Unphosphorylated ezrin, but not phosphorylated ezrin, was detected in COS7 cells, and ezrin silencing elevated Gag protein levels in the presence of VSV-G. Expression of unphosphorylated ezrin reduced Gag protein amounts. These results indicate that unphosphorylated ezrin proteins inhibit the VSV-G-mediated stabilization of HIV-1 Gag protein. Trafficking of HIV-1 Gag-associated intracellular vesicles may be controlled by ezrin. Finally, this study found that ezrin silencing yields higher amount of VSV-G-pseudotyped HIV-1 vector.", "subitem_description_type": "Abstract"}]}, "item_2_description_63": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "Frontiers in Bioengineering and Biotechnology, 8, art.no.368; 2020", "subitem_description_type": "Other"}]}, "item_2_publisher_33": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Frontiers Media S.A."}]}, "item_2_relation_12": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "10.3389/fbioe.2020.00368", "subitem_relation_type_select": "DOI"}}]}, "item_2_rights_13": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "c 2020 Izumida, Togawa, Hayashi, Matsuyama and Kubo. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted,provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms."}]}, "item_2_source_id_8": {"attribute_name": "EISSN", "attribute_value_mlt": [{"subitem_source_identifier": "22964185", "subitem_source_identifier_type": "ISSN"}]}, "item_2_version_type_16": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Izumida, Mai"}], "nameIdentifiers": [{"nameIdentifier": "3852", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Togawa, Kei"}], "nameIdentifiers": [{"nameIdentifier": "3853", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hayashi, Hideki"}], "nameIdentifiers": [{"nameIdentifier": "3854", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Matsuyama, Toshifumi"}], "nameIdentifiers": [{"nameIdentifier": "3855", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kubo, Yoshinao"}], "nameIdentifiers": [{"nameIdentifier": "3856", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-12-18"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "JBB8_368.pdf", "filesize": [{"value": "2.4 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 2400000.0, "url": {"label": "JBB8_368.pdf", "url": "https://nagasaki-u.repo.nii.ac.jp/record/823/files/JBB8_368.pdf"}, "version_id": "1021b333-a381-44ee-ae8d-458a99c28c29"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "HIV-1", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Gag protein", "subitem_subject_scheme": "Other"}, {"subitem_subject": "ezrin", "subitem_subject_scheme": "Other"}, {"subitem_subject": "phosphorylation", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Production of Vesicular Stomatitis Virus Glycoprotein-Pseudotyped Lentiviral Vector Is Enhanced by Ezrin Silencing", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Production of Vesicular Stomatitis Virus Glycoprotein-Pseudotyped Lentiviral Vector Is Enhanced by Ezrin Silencing"}]}, "item_type_id": "2", "owner": "2", "path": ["36"], "permalink_uri": "http://hdl.handle.net/10069/40008", "pubdate": {"attribute_name": "公開日", "attribute_value": "2020-05-29"}, "publish_date": "2020-05-29", "publish_status": "0", "recid": "823", "relation": {}, "relation_version_is_last": true, "title": ["Production of Vesicular Stomatitis Virus Glycoprotein-Pseudotyped Lentiviral Vector Is Enhanced by Ezrin Silencing"], "weko_shared_id": -1}
Production of Vesicular Stomatitis Virus Glycoprotein-Pseudotyped Lentiviral Vector Is Enhanced by Ezrin Silencing
http://hdl.handle.net/10069/40008
http://hdl.handle.net/10069/4000824cbbe8d-50fa-4298-94d3-fe9144853953
名前 / ファイル | ライセンス | アクション |
---|---|---|
JBB8_368.pdf (2.4 MB)
|
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2020-05-29 | |||||
タイトル | ||||||
タイトル | Production of Vesicular Stomatitis Virus Glycoprotein-Pseudotyped Lentiviral Vector Is Enhanced by Ezrin Silencing | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | HIV-1 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Gag protein | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ezrin | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | phosphorylation | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Izumida, Mai
× Izumida, Mai× Togawa, Kei× Hayashi, Hideki× Matsuyama, Toshifumi× Kubo, Yoshinao |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Human immunodeficiency virus type 1 (HIV-1)-based viral vector is widely used as a biomaterial to transfer a gene of interest into target cells in many biological study fields including gene therapy. Vesicular stomatitis virus glycoprotein (VSV-G)-containing HIV-1 vector much more efficiently transduces various mammalian cells than other viral envelope proteins-containing vectors. Understanding the mechanism would contribute to development of a novel method of efficient HIV-1 vector production. HIV-1 vector is generally constructed by transient transfection of human 293T or African green monkey COS7 cells. It was found in this study that HIV-1 Gag protein is constitutively digested in lysosomes of African green monkey cells. Surprisingly, VSV-G elevated HIV-1 Gag protein levels, suggesting that VSV-G protects Gag protein from the lysosomal degradation. Unphosphorylated ezrin, but not phosphorylated ezrin, was detected in COS7 cells, and ezrin silencing elevated Gag protein levels in the presence of VSV-G. Expression of unphosphorylated ezrin reduced Gag protein amounts. These results indicate that unphosphorylated ezrin proteins inhibit the VSV-G-mediated stabilization of HIV-1 Gag protein. Trafficking of HIV-1 Gag-associated intracellular vesicles may be controlled by ezrin. Finally, this study found that ezrin silencing yields higher amount of VSV-G-pseudotyped HIV-1 vector. | |||||
書誌情報 |
Frontiers in Bioengineering and Biotechnology 巻 8, p. 368, 発行日 2020-04-29 |
|||||
出版者 | ||||||
出版者 | Frontiers Media S.A. | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 22964185 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.3389/fbioe.2020.00368 | |||||
権利 | ||||||
権利情報 | c 2020 Izumida, Togawa, Hayashi, Matsuyama and Kubo. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted,provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Frontiers in Bioengineering and Biotechnology, 8, art.no.368; 2020 |