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Ocular Gene Delivery Systems Using Ternary Complexes of Plasmid DNA, Polyethylenimine, and Anionic Polymers
http://hdl.handle.net/10069/31453
http://hdl.handle.net/10069/3145352164585-3e36-4132-9d5e-5b7115627990
名前 / ファイル | ライセンス | アクション |
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BPBul36_96.pdf (2.8 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2013-04-22 | |||||
タイトル | ||||||
タイトル | Ocular Gene Delivery Systems Using Ternary Complexes of Plasmid DNA, Polyethylenimine, and Anionic Polymers | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Biomaterial | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | DNA | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Gene vector | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Nanoparticle | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Non-viral gene delivery | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Kurosaki, Tomoaki
× Kurosaki, Tomoaki× Uematsu, Masafumi× Shimoda, Kenichiro× Suzuma, Kiyoshi× Nakai, Masato× Nakamura, Tadahiro× Kitahara, Takashi× Kitaoka, Takashi× Sasaki, Hitoshi |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | In this experiment, we developed anionic ternary complexes for effective and safe ocular gene delivery. Ternary complexes were constructed by coating plasmid DNA (pDNA)/polyethylenimine (PEI) complex with anionic polymers such as γ-polyglutamic acid (γ-PGA) and chondroitin sulfate (CS). The cationic pDNA/PEI complex showed high gene expression on the human retinal pigment epithelial cell line, ARPE-19 cells. The pDNA/PEI complexes, however, also showed high cytotoxicity on the cells and aggregated strongly in the vitreous body. On the other hand, the anionic ternary complexes showed high gene expression on ARPE-19 cells without such cytotoxicity and aggregation. After intravitreous administration of the complexes, the anionic ternary complexes showed high gene expression in the retina. These results strongly indicate that anionic ternary complexes are suitable for effective and safe ocular gene therapy. | |||||
書誌情報 |
Biological and Pharmaceutical Bulletin 巻 36, 号 1, p. 96-101, 発行日 2013-01 |
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出版者 | ||||||
出版者 | 日本薬学会 | |||||
出版者別言語 | ||||||
Pharmaceutical Society of Japan | ||||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 09186158 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13475215 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1248/bpb.b12-00728 | |||||
権利 | ||||||
権利情報 | © 2013 The Pharmaceutical Society of Japan | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Biological and Pharmaceutical Bulletin, 36(1), pp.96-101; 2013 |