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Identification of bacteria directly from positive blood culture samples by DNA pyrosequencing of the 16S rRNA gene
http://hdl.handle.net/10069/31701
http://hdl.handle.net/10069/31701f2970ce5-3196-46db-915b-79c03d2ff157
名前 / ファイル | ライセンス | アクション |
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JMM61_1556.pdf (251.1 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2013-12-01 | |||||
タイトル | ||||||
タイトル | Identification of bacteria directly from positive blood culture samples by DNA pyrosequencing of the 16S rRNA gene | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | RNA 16S | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | article | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | bacterium detection | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | bacterium identification | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | bacterium isolate | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | blood culture | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | DNA determination | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | human | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | major clinical study | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | nonhuman | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | phenotype | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | polymerase chain reaction | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | priority journal | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | pyrosequencing | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | species identification | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Bacteria | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Bacterial Infections | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Bacteriological Techniques | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Base Sequence | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Candida albicans | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Candidiasis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Diphosphates | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | DNA, Bacterial | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | DNA, Fungal | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | DNA, Ribosomal | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Gene Expression Regulation, Bacterial | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Humans | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Polymerase Chain Reaction | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | RNA, Bacterial | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | RNA, Ribosomal, 16S | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Sepsis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Sequence Analysis, DNA | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Species Specificity | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Motoshima, Maiko
× Motoshima, Maiko× Yanagihara, Katsunori× Morinaga, Yoshitomo× Matsuda, Junichi× Hasegawa, Hiroo× Kohno, Shigeru× Kamihira, Shimeru |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Rapid identification of the causative bacteria of sepsis in patients can contribute to the selection of appropriate antibiotics and improvement of patients' prognosis. Genotypic identification is an emerging technology that may provide an alternative method to, or complement, established phenotypic identification procedures. We evaluated a rapid protocol for bacterial identification based on PCR and pyrosequencing of the V1 and V3 regions of the 16S rRNA gene using DNA extracted directly from positive blood culture samples. One hundred and two positive blood culture bottles from 68 patients were randomly selected and the bacteria were identified by phenotyping and pyrosequencing. The results of pyrosequencing identification displayed 84.3 and 64.7% concordance with the results of phenotypic identification at the genus and species levels, respectively. In the monomicrobial samples, the concordance between the results of pyrosequencing and phenotypic identification at the genus level was 87.0 %. Pyrosequencing identified one isolate in 60% of polymicrobial samples, which were confirmed by culture analysis. Of the samples identified by pyrosequencing, 55.7% showed consistent results in V1 and V3 targeted sequencing; other samples were identified based on the results of V1 (12.5 %) or V3 (31.8 %) sequencing alone. One isolate was erroneously identified by pyrosequencing due to high sequence similarity with another isolate. Pyrosequencing identified one isolate that was not detected by phenotypic identification. The process of pyrosequencing identification can be completed within ~4 h. The information provided by DNA-pyrosequencing for the identification of micro-organisms in positive blood culture bottles is accurate and could prove to be a rapid and useful tool in standard laboratory practice. | |||||
書誌情報 |
Journal of Medical Microbiology 巻 61, 号 11, p. 1556-1562, 発行日 2012-11 |
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出版者 | ||||||
出版者 | Society for General Microbiology | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00222615 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 14735644 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1099/jmm.0.049163-0 | |||||
権利 | ||||||
権利情報 | © 2012 SGM. | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Journal of Medical Microbiology, 61(11), pp.1556-1562; 2012 |