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Improved membrane fluidity of ionic polysaccharide bead-supported phospholipid bilayer membrane systems
http://hdl.handle.net/10069/31767
http://hdl.handle.net/10069/31767ccf0fdec-fcd4-4101-9372-e3d723ab8947
名前 / ファイル | ライセンス | アクション |
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CSB107_90.pdf (5.8 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2013-05-15 | |||||
タイトル | ||||||
タイトル | Improved membrane fluidity of ionic polysaccharide bead-supported phospholipid bilayer membrane systems | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | FRAP | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Membrane fluidity | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Phospholipid | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Polysaccharide | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Supported lipid membrane | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Haratake, Mamoru
× Haratake, Mamoru× Takahira, Ekuko× Yoshida, Sakura× Osei-Asante, Samuel× Fuchigami, Takeshi× Nakayama, Morio |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Supported phospholipid bilayer membranes on polysaccharide-based cationic polymer beads (cationic group: -[OCH2CH(OH)CH2]2N+(CH3)3·X-, 45-165μm in diameter) were prepared using small unilamellar vesicles from mixtures of phosphatidylserine (PS) and phosphatidylcholine (PC). Confocal fluorescence microscopic observations with a fluorescent membrane probe (N-4-nitrobenzo-2-oxa-1,3-diazole-phosphatidylethanolamine) revealed that the phospholipid molecules in the phospholipid-bead complexes were along the outer surface of the beads. The fluidity of the phospholipid bilayer membranes in the PS/PC-bead complexes was investigated by the fluorescence recovery after photobleaching (FRAP) technique. The lateral diffusion coefficients (D) for the PS/PC-bead complexes were lower than that for the 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine giant unilamellar vesicles without solid supports. Such less fluid membranes in the complexes appeared to be due to the immobilization of the phospholipid bilayer membranes by electrostatic attractive forces between PS and the bead. The D values for the PS/PC-bead complexes were dependent on the phospholipid composition; the PS(100mol%)/PC(0mol%)-bead complex had the least fluid membranes among the PS/PC-bead complexes tested in this study. The phospholipid bilayer membranes formed on the polysaccharide-based cationic polymer beads were much more fluid than those on a polystyrene-based one. Furthermore, such fluid phospholipid bilayer membranes formed on the polysaccharide-based cationic polymer bead were maintained for 10 days, even though the complex sample was stood in plain buffer (pH 8.5) at ambient temperature. | |||||
書誌情報 |
Colloids and Surfaces B: Biointerfaces 巻 107, p. 90-96, 発行日 2013-07-01 |
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出版者 | ||||||
出版者 | Elsevier | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 09277765 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.colsurfb.2013.01.070 | |||||
権利 | ||||||
権利情報 | © 2013 Elsevier B.V. | |||||
権利 | ||||||
権利情報 | NOTICE: this is the author’s version of a work that was accepted for publication in Colloids and Surfaces B: Biointerfaces. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Colloids and Surfaces B: Biointerfaces, 107(2013) | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Colloids and Surfaces B: Biointerfaces, 107, pp.90-96; 2013 |