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Feasibility studies of radioiodinated pyridyl benzofuran derivatives as potential SPECT imaging agents for prion deposits in the brain
http://hdl.handle.net/10069/40318
http://hdl.handle.net/10069/40318408d7938-e455-4715-9e1a-4a320970f4cc
名前 / ファイル | ライセンス | アクション |
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NMB90-91_41.pdf (971.3 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2020-12-16 | |||||
タイトル | ||||||
タイトル | Feasibility studies of radioiodinated pyridyl benzofuran derivatives as potential SPECT imaging agents for prion deposits in the brain | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Amyloid | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Prion diseases | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | PrPSc | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Pyridyl benzofurans | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Single photon emission computed tomography (SPECT) | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Fuchigami, Takeshi
× Fuchigami, Takeshi× Kawasaki, Masao× Watanabe, Hiroyuki× Nakagaki, Takehiro× Nishi, Kodai× Sano, Kazunori× Atarashi, Ryuichiro× Nakaie, Mari× Yoshida, Sakura× Ono, Masahiro× Nishida, Noriyuki× Nakayama, Morio |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Introduction: Prion diseases are fatal neurodegenerative disorders caused by the deposition of abnormal prion protein aggregates (PrPSc) in the central nervous system. This study aimed to evaluate the use of iodinated pyridyl benzofuran(IPBF) derivatives as single-photon emission computed tomography (SPECT) probes for the detection of cerebral PrPSc deposits. Methods: In vitro binding assays of IPBF derivatives were carried out in the recombinant mouse prion protein (rMoPrP) and brain sections of mouse-adapted bovine spongiform encephalopathy (mBSE)-infected mice. SPECT imaging of 5-(5-[123I] iodobenzofuran-2-yl)-N-methylpyridin-2-amine ([123I]IPBF-NHMe) was performed on mBSE-infected and mock-infected mice. Results: Fluorescence microscopy results showed that fluorescence signals of IPBF derivatives corresponded to the thiof lavin-T positive amyloid deposits of PrPSc in the brain sections of mouse-adapted bovine spongiform encephalopathy (mBSE)-infected mice. Among the IPBF derivatives, 5-(5-iodobenzofuran-2-yl)-N-methylpyridin-2-amine (IPBF-NHMe) exhibited the highest binding affinity to the recombinant mouse prion protein (rMoPrP) aggregates with a Ki of 14.3 nM. SPECT/computed tomography (CT) imaging and ex vivo autoradiography demonstrated that the [123I]IPBF-NHMe distribution in brain tissues of mBSE-infected mice co-localized with PrPSc deposits. Conclusion: [123I]IPBF-NHMe appears to be a prospective SPECT tracer for monitoring prion deposits in living brain tissues. | |||||
書誌情報 |
Nuclear Medicine and Biology 巻 90-91, p. 41-48, 発行日 2020-09-15 |
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出版者 | ||||||
出版者 | Elsevier Inc. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 09698051 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.nucmedbio.2020.09.003 | |||||
権利 | ||||||
権利情報 | c 2020 Elsevier Inc. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Nuclear Medicine and Biology, 90-91, pp.41-48; 2020 |