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Discrimination based on Gly and Arg/Ser at position 673 between dipeptidyl-peptidase (DPP) 7 and DPP11, widely distributed DPPs in pathogenic and environmental gram-negative bacteria
http://hdl.handle.net/10069/30455
http://hdl.handle.net/10069/30455faff718d-e4e8-4017-bd35-7c67e83a7b54
名前 / ファイル | ライセンス | アクション |
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Biochi95_824.pdf (846.1 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2013-01-31 | |||||
タイトル | ||||||
タイトル | Discrimination based on Gly and Arg/Ser at position 673 between dipeptidyl-peptidase (DPP) 7 and DPP11, widely distributed DPPs in pathogenic and environmental gram-negative bacteria | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Dipeptidyl peptidase | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | S46 peptidase | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Substrate specificity | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Porphyromonas gingivalis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Bacteroidetes | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Proteobacteria | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Rouf, Shakh M.A.
× Rouf, Shakh M.A.× Ohara-Nemoto, Yuko× Hoshino, Tomonori× Fujiwara, Taku× Ono, Toshio× Nemoto, Takayuki K. |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Porphyromonas gingivalis, an asaccharolytic gram-negative rod-shaped bacterium, expresses the novel Asp/Glu-specific dipeptidyl-peptidase (DPP) 11 (Ohara-Nemoto, Y. et al. (2011) J. Biol. Chem. 286, 38115–38127), which has been categorized as a member of the S46/DPP7 family that is preferential for hydrophobic residues at the P1 position. From that finding, 129 gene products constituting five clusters from the phylum Bacteroidetes have been newly annotated to either DPP7 or DPP11, whereas the remaining 135 members, mainly from the largest phylum Proteobacteria, have yet to be assigned. In this study, the substrate specificities of the five clusters and an unassigned group were determined with recombinant DPPs from typical species, i.e., P. gingivalis, Capnocytophaga gingivalis, Flavobacterium psychrophilum, Bacteroides fragilis, Bacteroides vulgatus, and Shewanella putrefaciens. Consequently, clusters 1, 3, and 5 were found to be DPP7 with rather broad substrate specificity, and clusters 2 and 4 were DPP11. An unassigned S. putrefaciens DPP carrying Ser673 exhibited Asp/Glu-specificity more preferable to Glu, in contrast to the Asp preference of DPP11 with Arg673 from Bacteroidetes species. Mutagenesis experiments revealed that Arg673/Ser673 were indispensable for the Asp/Glu-specificity of DPP11, and that the broad specificity of DPP7 was mediated by Gly673. Taken together with the distribution of the two genes, all 264 members of the S46 family could be attributed to either DPP7 or DPP11 by an amino acid at position 673. A more compelling phylogenic tree based on the conserved C-terminal region suggested two gene duplication events in the phylum Bacteroidetes, one causing the development of DPP7 and DPP11 with altered substrate specificities, and the other producing an additional DPP7 in the genus Bacteroides. | |||||
書誌情報 |
Biochimie 巻 95, 号 4, p. 824-832, 発行日 2012 |
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出版者 | ||||||
出版者 | Elsevier Masson SAS | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 03009084 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.biochi.2012.11.019 | |||||
権利 | ||||||
権利情報 | © 2012 Elsevier Masson SAS. All rights reserved. | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
関係URI | ||||||
関連名称 | http://hdl.handle.net/10069/32711 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Biochimie, 95(4), pp.824-832; 2013 |