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Comparative study on antioxidative and macrophage-stimulating activities of polyguluronic acid (PG) and polymannuronic acid (PM) prepared from alginate
http://hdl.handle.net/10069/28904
http://hdl.handle.net/10069/289040a144cd3-9b50-4cf0-84a3-d707a5b1331d
名前 / ファイル | ライセンス | アクション |
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CarRes352_88.pdf (776.2 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2012-08-01 | |||||
タイトル | ||||||
タイトル | Comparative study on antioxidative and macrophage-stimulating activities of polyguluronic acid (PG) and polymannuronic acid (PM) prepared from alginate | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Alginate oligomer | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Polyguluronic acid | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Polymannuronic acid | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Antioxidative activity | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Nitric oxide | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Ueno, Mikinori
× Ueno, Mikinori× Hiroki, Taichi× Takeshita, Satoshi× Jiang, Zedong× Kim, Daekyung× Yamaguchi, Kenichi× Oda, Tatsuya |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The antioxidant and macrophage-stimulating activities of polyguluronic acid (PG) and polymannuronic acid (PM) prepared from alginate were examined. A chemiluminescence (CL) method using a luminol analog, L-012, showed that both PM and PG scavenge superoxide produced by hypoxanthine–xanthine oxidase system in a concentration-dependent manner. At 100 μg/ml, PG showed slightly stronger superoxide scavenging activity than PM. In an electron spin resonance (ESR)-spin trapping method in which the Fenton reaction was used as hydroxyl radical generation system, we found that both PM and PG showed potent hydroxyl radical scavenging activity to a similar extent. Because PM and PG showed no chelating activity on Fe2+, it was confirmed that PM and PG can directly scavenge hydroxyl radical. No significant scavenging activity of PM and PG toward hydrogen peroxide was observed. Interestingly, the macrophage-stimulation activity of PG as measured by nitric oxide (NO)-production from mouse macrophage cell line RAW264.7 cells was evidently stronger than that of PM. Our results suggest that RAW264.7 cells might be able to distinguish the conformational differences between PM and PG, and respond differently to them, whereas the effects of such structural differences between PM and PG on the radical scavenging activities may not be so significant. | |||||
書誌情報 |
Carbohydrate Research 巻 352, p. 88-93, 発行日 2012-05-01 |
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出版者 | ||||||
出版者 | Elsevier Ltd. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00086215 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.carres.2012.02.005 | |||||
権利 | ||||||
権利情報 | © Elsevier Ltd. | |||||
権利 | ||||||
権利情報 | NOTICE: this is the author’s version of a work that was accepted for publication in Carbohydrate Research. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Carbohydrate Research, 352(2012) | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Carbohydrate Research, 352, pp.88-93; 2012 |