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アイテム
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Live Cell Labeling with Terpyridine Derivative Proligands to Measure Cytotoxicity Mediated by Immune Cells
http://hdl.handle.net/10069/38318
http://hdl.handle.net/10069/38318038200c7-70c1-4dde-abde-782e2b986c37
名前 / ファイル | ライセンス | アクション |
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ChemMedChem12_2006.pdf (1.7 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2018-12-07 | |||||
タイトル | ||||||
タイトル | Live Cell Labeling with Terpyridine Derivative Proligands to Measure Cytotoxicity Mediated by Immune Cells | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | cancer immunotherapy | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | cytotoxicity assays | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | NK cells | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | T cells | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | terpyridine proligands | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Sakai, Yuki
× Sakai, Yuki× Mizuta, Satoshi× Kumagai, Asuka× Tagod, Mohammed S. O.× Senju, Hiroaki× Nakamura, Tatsufumi× Morita, Craig T.× Tanaka, Yoshimasa |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Immunotherapy using immune checkpoint inhibitors and CAR-T cells has revolutionized treatment for patients with malignant tumors. However, measuring tumor cell cytotoxicity mediated by immune effector cells in clinical laboratories has been difficult due to the requirement for radioactive substances. In this study, a series of novel terpyridine derivative proligands were synthesized, and a non-radioactive cellular cytotoxicity assay using the newly synthesized compounds was developed for use in preclinical and clinical studies for cancer immunotherapy. Once internalized into target cells, the compounds are hydrolyzed by esterases, resulting in the intracellular accumulation of the negatively charged terpyridine derivatives. When the labeled target cells are recognized and killed by immune effector cells, the integrity of the cell membrane is disrupted, and the terpyridine derivatives are released. Upon combining the culture supernatant with europium (Eu3+), the cytotoxicity of immune effector cells for the target cells can be quantitatively determined by measuring the intensity of the Eu3+/ligand-derived time-resolved fluorescence. Thus, the assay developed in this study would facilitate the development of novel cancer immunotherapies. | |||||
書誌情報 |
ChemMedChem 巻 12, 号 23, p. 2006-2013, 発行日 2017-12-07 |
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出版者 | ||||||
出版者 | Wiley-VCH Verlag GmbH & Co. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 18607179 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1002/cmdc.201700626 | |||||
権利 | ||||||
権利情報 | c 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim | |||||
権利 | ||||||
権利情報 | This is the peer reviewed version of the following article: ChemMedChemVolume, 12(23), pp.2006-2013; 2017, which has been published in final form at http://dx.doi.org/10.1002/cmdc.201700626. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. |
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著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | ChemMedChem, 12(23), pp.2006-2013; 2017 |