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Confocal fluorescent microscopy showed that riboflavin was mainly localized in the epidermis and cortex of the root tip and, at the cellular level, in the apoplast. The expressions of three genes involved in the de novo biosynthesis of riboflavin (GTP cyclohydrolase II/3,4-dihydroxy-2-butanone 4-phosphate synthase; 6,7-dimethyl-8-ribityllumazine synthase; riboflavin synthase) were compared between Fe-starved and Fe-replete roots over a time-course of 7 days, using RT-PCR. All three genes were found to be highly expressed over the period 1–7 days in the roots cultured under Fe deficiency. Since riboflavin secretion began to be detected only from 3 days, there was a lag phase observed between the increased transcript accumulations and riboflavin secretion. 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Increased de novo riboflavin synthesis and hydrolysis of FMN are involved in riboflavin secretion from Hyoscyamus albus hairy roots under iron deficiency
http://hdl.handle.net/10069/29350
http://hdl.handle.net/10069/293503a25a2cd-a33c-4281-afb9-559694328ffa
名前 / ファイル | ライセンス | アクション |
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PPB58_166.pdf (754.1 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2012-10-01 | |||||
タイトル | ||||||
タイトル | Increased de novo riboflavin synthesis and hydrolysis of FMN are involved in riboflavin secretion from Hyoscyamus albus hairy roots under iron deficiency | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Hyoscyamus albus | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Hairy roots | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Iron deficiency | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Riboflavin secretion | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Riboflavin biosynthesis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | FMN hydrolase | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Respiration | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Higa, Ataru
× Higa, Ataru× Khandakar, Jebunnahar× Mori, Yuko× Kitamura, Yoshie |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Riboflavin secretion by Hyoscyamus albus hairy roots under Fe deficiency was examined to determine where riboflavin is produced and whether production occurs via an enhancement of riboflavin biosynthesis or a stimulation of flavin mononucleotide (FMN) hydrolysis. Confocal fluorescent microscopy showed that riboflavin was mainly localized in the epidermis and cortex of the root tip and, at the cellular level, in the apoplast. The expressions of three genes involved in the de novo biosynthesis of riboflavin (GTP cyclohydrolase II/3,4-dihydroxy-2-butanone 4-phosphate synthase; 6,7-dimethyl-8-ribityllumazine synthase; riboflavin synthase) were compared between Fe-starved and Fe-replete roots over a time-course of 7 days, using RT-PCR. All three genes were found to be highly expressed over the period 1–7 days in the roots cultured under Fe deficiency. Since riboflavin secretion began to be detected only from 3 days, there was a lag phase observed between the increased transcript accumulations and riboflavin secretion. To determine whether FMN hydrolysis might contribute to the riboflavin secretion in Fe-deficient root cultures, FMN hydrolase activity was determined and was found to be substantially increased after 3 days, when riboflavin secretion became detectable. These results suggested that not only de novo riboflavin synthesis but also the hydrolysis of FMN contributes to riboflavin secretion under conditions of Fe deficiency. Respiration activity was assayed during the time-course, and was also found to be enhanced after 3 days under Fe deficiency, suggesting a possible link with riboflavin secretion. On the other hand, several respiratory inhibitors were found not to affect riboflavin synthase transcript accumulation. | |||||
書誌情報 |
Plant Physiology and Biochemistry 巻 58, p. 166-173, 発行日 2012-09 |
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出版者 | ||||||
出版者 | Elsevier Masson SAS. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 18732690 | |||||
PubMed番号 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | PMID | |||||
関連識別子 | 22819862 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.plaphy.2012.07.001 | |||||
権利 | ||||||
権利情報 | © 2012 Elsevier Masson SAS. All rights reserved. | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Plant Physiology and Biochemistry, 58, pp.166-173; 2012 |