WEKO3
アイテム
{"_buckets": {"deposit": "cec37bb4-0403-424e-ba80-b4cdfa050a3e"}, "_deposit": {"created_by": 2, "id": "10840", "owners": [2], "pid": {"revision_id": 0, "type": "depid", "value": "10840"}, "status": "published"}, "_oai": {"id": "oai:nagasaki-u.repo.nii.ac.jp:00010840", "sets": ["13"]}, "author_link": ["42562", "42566", "42571", "42563", "42564", "42567", "42558", "42565", "42569", "42570", "42557", "42568", "42559", "42561", "42560"], "item_2_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2012-03-28", "bibliographicIssueDateType": "Issued"}, "bibliographicPageStart": "92", "bibliographicVolumeNumber": "11", "bibliographic_titles": [{"bibliographic_title": "Malaria Journal"}]}]}, "item_2_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Background. In Plasmodium falciparum, resistance to chloroquine (CQ) is conferred by a K to T mutation at amino acid position 76 (K76T) in the P. falciparum CQ transporter (PfCRT). To date, at least 15 pfcrt genotypes, which are represented by combinations of five amino acids at positions 72-76, have been described in field isolates from various endemic regions. To identify novel mutant pfcrt genotypes and to reveal the genetic relatedness of pfcrt genotypes, a large-scale survey over a wide geographic area was performed. Methods. Sequences for exon 2 in pfcrt, including known polymorphic sites at amino acid positions 72, 74, 75 and 76, were obtained from 256 P. falciparum isolates collected from eight endemic countries in Asia (Bangladesh, Cambodia, Lao P.D.R., the Philippines and Thailand), Melanesia (Papua New Guinea and Vanuatu) and Africa (Ghana). A haplotype network was constructed based on six microsatellite markers located -29 kb to 24 kb from pfcrt in order to examine the genetic relatedness among mutant pfcrt genotypes. Results. In addition to wild type (CVMNK at positions 72-76), four mutant pfcrt were identified; CVIET, CVIDT, SVMNT and CVMNT (mutated amino acids underlined). Haplotype network revealed that there were only three mutant pfcrt lineages, originating in Indochina, Philippines and Melanesia. Importantly, the Indochina lineage contained two mutant pfcrt genotypes, CVIET (n = 95) and CVIDT (n = 14), indicating that CVIDT shares a common origin with CVIET. Similarly, one major haplotype in the Melanesian lineage contained two pfcrt genotypes; SVMNT (n = 71) and CVMNT (n = 3). In Africa, all mutant pfcrt genotypes were the CVIET of the Indochina lineage, probably resulting from the intercontinental migration of CQ resistance from Southeast Asia. Conclusions. The number of CQ-mutant lineages observed in this study was identical to that found in previous studies. This supports the hypothesis that the emergence of novel CQ resistance is rare. However, in the mutant pfcrt genotypes, amino acid changes at positions 72, 74 and 75 appear to have recently been generated at least several times, producing distinct pfcrt mutant genotypes. The occurrence of new mutations flanking K76T may yield stronger resistance to CQ and/or a higher fitness than the original pfcrt mutant.", "subitem_description_type": "Abstract"}]}, "item_2_description_63": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "Malaria Journal, 11, no92; 2012", "subitem_description_type": "Other"}]}, "item_2_publisher_33": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "BioMed Central"}]}, "item_2_relation_12": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1186/1475-2875-11-9222453078", "subitem_relation_type_select": "DOI"}}]}, "item_2_rights_13": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "© 2012 Takahashi et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative\nCommons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and\nreproduction in any medium, provided the original work is properly cited."}]}, "item_2_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "14752875", "subitem_source_identifier_type": "ISSN"}]}, "item_2_version_type_16": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Takahashi, Nobuyuki"}], "nameIdentifiers": [{"nameIdentifier": "42557", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tanabe, Kazuyuki"}], "nameIdentifiers": [{"nameIdentifier": "42558", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tsukahara, Takahiro"}], "nameIdentifiers": [{"nameIdentifier": "42559", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Dzodzomenyo, Mawuli"}], "nameIdentifiers": [{"nameIdentifier": "42560", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Dysoley, Lek"}], "nameIdentifiers": [{"nameIdentifier": "42561", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Khamlome, Boualam"}], "nameIdentifiers": [{"nameIdentifier": "42562", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sattabongkot, Jetsumon"}], "nameIdentifiers": [{"nameIdentifier": "42563", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nakamura, Masatoshi"}], "nameIdentifiers": [{"nameIdentifier": "42564", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sakurai, Miki"}], "nameIdentifiers": [{"nameIdentifier": "42565", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kobayashi, Jun"}], "nameIdentifiers": [{"nameIdentifier": "42566", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kaneko, Akira"}], "nameIdentifiers": [{"nameIdentifier": "42567", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Endo, Hiroyoshi"}], "nameIdentifiers": [{"nameIdentifier": "42568", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hombhanje, Francis"}], "nameIdentifiers": [{"nameIdentifier": "42569", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tsuboi, Takafumi"}], "nameIdentifiers": [{"nameIdentifier": "42570", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Mita, Toshihiro"}], "nameIdentifiers": [{"nameIdentifier": "42571", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-12-22"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "MalJou11_92.pdf", "filesize": [{"value": "565.7 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 565700.0, "url": {"label": "MalJou11_92.pdf", "url": "https://nagasaki-u.repo.nii.ac.jp/record/10840/files/MalJou11_92.pdf"}, "version_id": "50f301fd-0083-465a-93cb-9d8eb4b6704e"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Chloroquine resistance", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Evolution", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Haplotype network", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Microsatellite", "subitem_subject_scheme": "Other"}, {"subitem_subject": "pfcrt", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Plasmodium falciparum", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Large-scale survey for novel genotypes of Plasmodium falciparum chloroquine-resistance gene pfcrt", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Large-scale survey for novel genotypes of Plasmodium falciparum chloroquine-resistance gene pfcrt"}]}, "item_type_id": "2", "owner": "2", "path": ["13"], "permalink_uri": "http://hdl.handle.net/10069/29401", "pubdate": {"attribute_name": "公開日", "attribute_value": "2012-10-09"}, "publish_date": "2012-10-09", "publish_status": "0", "recid": "10840", "relation": {}, "relation_version_is_last": true, "title": ["Large-scale survey for novel genotypes of Plasmodium falciparum chloroquine-resistance gene pfcrt"], "weko_shared_id": -1}
Large-scale survey for novel genotypes of Plasmodium falciparum chloroquine-resistance gene pfcrt
http://hdl.handle.net/10069/29401
http://hdl.handle.net/10069/2940174056720-98cf-4a31-ba87-e941efc5119e
名前 / ファイル | ライセンス | アクション |
---|---|---|
MalJou11_92.pdf (565.7 kB)
|
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2012-10-09 | |||||
タイトル | ||||||
タイトル | Large-scale survey for novel genotypes of Plasmodium falciparum chloroquine-resistance gene pfcrt | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Chloroquine resistance | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Evolution | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Haplotype network | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Microsatellite | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | pfcrt | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Plasmodium falciparum | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Takahashi, Nobuyuki
× Takahashi, Nobuyuki× Tanabe, Kazuyuki× Tsukahara, Takahiro× Dzodzomenyo, Mawuli× Dysoley, Lek× Khamlome, Boualam× Sattabongkot, Jetsumon× Nakamura, Masatoshi× Sakurai, Miki× Kobayashi, Jun× Kaneko, Akira× Endo, Hiroyoshi× Hombhanje, Francis× Tsuboi, Takafumi× Mita, Toshihiro |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Background. In Plasmodium falciparum, resistance to chloroquine (CQ) is conferred by a K to T mutation at amino acid position 76 (K76T) in the P. falciparum CQ transporter (PfCRT). To date, at least 15 pfcrt genotypes, which are represented by combinations of five amino acids at positions 72-76, have been described in field isolates from various endemic regions. To identify novel mutant pfcrt genotypes and to reveal the genetic relatedness of pfcrt genotypes, a large-scale survey over a wide geographic area was performed. Methods. Sequences for exon 2 in pfcrt, including known polymorphic sites at amino acid positions 72, 74, 75 and 76, were obtained from 256 P. falciparum isolates collected from eight endemic countries in Asia (Bangladesh, Cambodia, Lao P.D.R., the Philippines and Thailand), Melanesia (Papua New Guinea and Vanuatu) and Africa (Ghana). A haplotype network was constructed based on six microsatellite markers located -29 kb to 24 kb from pfcrt in order to examine the genetic relatedness among mutant pfcrt genotypes. Results. In addition to wild type (CVMNK at positions 72-76), four mutant pfcrt were identified; CVIET, CVIDT, SVMNT and CVMNT (mutated amino acids underlined). Haplotype network revealed that there were only three mutant pfcrt lineages, originating in Indochina, Philippines and Melanesia. Importantly, the Indochina lineage contained two mutant pfcrt genotypes, CVIET (n = 95) and CVIDT (n = 14), indicating that CVIDT shares a common origin with CVIET. Similarly, one major haplotype in the Melanesian lineage contained two pfcrt genotypes; SVMNT (n = 71) and CVMNT (n = 3). In Africa, all mutant pfcrt genotypes were the CVIET of the Indochina lineage, probably resulting from the intercontinental migration of CQ resistance from Southeast Asia. Conclusions. The number of CQ-mutant lineages observed in this study was identical to that found in previous studies. This supports the hypothesis that the emergence of novel CQ resistance is rare. However, in the mutant pfcrt genotypes, amino acid changes at positions 72, 74 and 75 appear to have recently been generated at least several times, producing distinct pfcrt mutant genotypes. The occurrence of new mutations flanking K76T may yield stronger resistance to CQ and/or a higher fitness than the original pfcrt mutant. | |||||
書誌情報 |
Malaria Journal 巻 11, p. 92, 発行日 2012-03-28 |
|||||
出版者 | ||||||
出版者 | BioMed Central | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 14752875 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1186/1475-2875-11-9222453078 | |||||
権利 | ||||||
権利情報 | © 2012 Takahashi et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
|||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Malaria Journal, 11, no92; 2012 |