WEKO3
アイテム
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Estrogen-dependent regulation of sodium/hydrogen exchanger-3 (NHE3) expression via estrogen receptor β in proximal colon of pregnant mice
http://hdl.handle.net/10069/29497
http://hdl.handle.net/10069/29497b993c34e-28b5-4812-b1b0-7a625318402e
名前 / ファイル | ライセンス | アクション |
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HCB137_575.pdf (937.2 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2012-10-24 | |||||
タイトル | ||||||
タイトル | Estrogen-dependent regulation of sodium/hydrogen exchanger-3 (NHE3) expression via estrogen receptor β in proximal colon of pregnant mice | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Constipation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Estrogen receptor β | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | NHE3 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Pregnancy | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Proximal colon | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Choijookhuu, Narantsog
× Choijookhuu, Narantsog× Sato, Yoko× Nishino, Tomoya× Endo, Daisuke× Hishikawa, Yoshitaka× Koji, Takehiko |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Although constipation is very common during pregnancy, the exact mechanism is unknown. We hypothesized that the involvement of estrogen receptor (ER) in the regulation of electrolyte transporter in the colon leads to constipation. In this study, the intestines of normal female ICR mouse and pregnant mice were examined for the expression of ERβ and ERβ by immunohistochemistry and in situ hybridization. ERα, but not ERα, was expressed in surface epithelial cells of the proximal, but not distal, colon on pregnancy days 10, 15, and 18, but not day 5, and the number of ERα-positive cells increased signiWcantly during pregnancy. Expression of NHE3, the gene that harbors estrogen response element, examined by immunohistochemistry and western blotting, was localized in the surface epithelial cells of the proximal colon and increased in parallel with ERβ expression. In ovariectomized mice, NHE3 expression was only marginal and was up-regulated after treatment with 17- estradiol (E2), but not E 2 + ICI 182,780 (estrogen receptor antagonist). Moreover, knock-down of ERβ expression by electroporetically transfected siRNA resulted in a signiWcant reduction of NHE3 expression. These results indicate that ERβ regulates the expression of NHE3 in the proximal colon of pregnant mice through estrogen action, suggesting the involvement of increased sodium absorption by up-regulated NHE3 in constipation during pregnancy. | |||||
書誌情報 |
Histochemistry and Cell Biology 巻 137, 号 5, p. 575-587, 発行日 2012-05 |
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出版者 | ||||||
出版者 | Springer Verlag | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 09486143 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1432119X | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1007/s00418-012-0935-2 | |||||
権利 | ||||||
権利情報 | © 2012 Springer-Verlag. | |||||
権利 | ||||||
権利情報 | The final publication is available at www.springerlink.com. | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
関係URI | ||||||
関連名称 | http://hdl.handle.net/10069/28767 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Histochemistry and Cell Biology, 137(5), pp.575-587; 2012 |