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Inhibitory effect of sulphated polysaccharide porphyran on nitric oxide production in lipopolysaccharide-stimulated RAW264.7 macrophages
http://hdl.handle.net/10069/27444
http://hdl.handle.net/10069/2744467b8cbc8-a4b6-47b1-a215-76df15e3e24e
名前 / ファイル | ライセンス | アクション |
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JouBio151_65.pdf (219.4 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2013-02-01 | |||||
タイトル | ||||||
タイトル | Inhibitory effect of sulphated polysaccharide porphyran on nitric oxide production in lipopolysaccharide-stimulated RAW264.7 macrophages | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | iNOS | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | NF-κB | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | nitric oxide | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | porphyran | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | sulphated polysaccharide | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Jiang, Zedong
× Jiang, Zedong× Hama, Yoichiro× Yamaguchi, Kenichi× Oda, Tatsuya |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Porphyran, extracted from an edible red alga (Porphyra yezoensis), is a sulphated polysaccharide with a wide variety of biological activities including anti-tumour, antioxidant and immuno-modulating activities. In this study, we examined the effect of porphyran on nitric oxide (NO) production in mouse macrophage cell line RAW264.7 cells. Although no significant activity of porphyran to induce NO or tumour necrosis factor-α (TNF-α) production in RAW264.7 cells was observed at the concentration range tested (10-500 g/ml), it was found for the first time that porphyran inhibited NO production and expression of inducible nitric oxide synthase (iNOS) in RAW264.7 cells stimulated with lipopolysaccharide (LPS). In the presence of 500 g/ml porphyran, NO production and expression of iNOS in LPS-treated RAW264.7 cells were completely suppressed. On the other hand, porphyran showed only a marginal effect on the secretion of TNF-α from LPS-stimulated RAW264.7 cells. Electrophoretic mobility shift assay (EMSA) using infrared dye labelled oligonucleotide with nuclear factor-κB (NF-κB) consensus sequence suggested that porphyran inhibited the LPS-induced NF-κB activation. The LPS-inducible nuclear translocation of p65, and the phosphorylation and degradation of IκB-α were also inhibited by the pre-treatment with porphyran. Our results obtained in in vitro analysis suggest that porphyran suppresses NO production in LPS-stimulated macrophages by the blocking of NF-κB activation. | |||||
書誌情報 |
Journal of Biochemistry 巻 151, 号 1, p. 65-74, 発行日 2012-01 |
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出版者 | ||||||
出版者 | Oxford University Press | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0021924X | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00694073 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1093/jb/mvr115 | |||||
権利 | ||||||
権利情報 | © The Authors 2011. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved | |||||
権利 | ||||||
権利情報 | This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Journal of Biochemistry following peer review. The definitive publisher-authenticated version Journal of Biochemistry, 151(1), pp.65-74; 2012 is available online at: http://dx.doi.org/10.1093/jb/mvr115. | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Journal of Biochemistry, 151(1), pp.65-74; 2012 |