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HPLC-UV method development for fentanyl determination in rat plasma and its application to elucidate pharmacokinetic behavior after i.p. administration to rats.
http://hdl.handle.net/10069/26235
http://hdl.handle.net/10069/26235c5b29089-942e-4f38-9c3b-07bf60c07a35
名前 / ファイル | ライセンス | アクション |
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JCB879_2941.pdf (346.5 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2011-11-04 | |||||
タイトル | ||||||
タイトル | HPLC-UV method development for fentanyl determination in rat plasma and its application to elucidate pharmacokinetic behavior after i.p. administration to rats. | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Deproteinization | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Fentanyl | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | High performance liquid chromatography | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Pharmacokinetics | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Rat plasma | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | UV detection | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Almousa, Ahmed A
× Almousa, Ahmed A× Ikeda, Rie× Wada, Mitsuhiro× Kuroda, Naotoka× Hanajiri, Ruri-Kikura× Nakashima, Kenichiro |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | A simple, rapid and validated high performance liquid chromatography method with UV detection for the quantification of an opioid agonist, fentanyl (FEN), in rat plasma was developed. The assay procedure involved chromatographic separation using a ZIC-HILIC SeQUANT column (250 mm × 4.6 mm, i.d., 5 μm) and a mobile phase of acetonitrile and acetate buffer (pH 3.4, 20mM) of ratio (=65:35, v/v) at a flow rate of 1.2 mL/min and detection wavelength of 201 nm. Plasma sample (100 μL) pretreatment was based on simple deprotienization by acetonitrile spiked with clonidine as an internal standard (I.S.) of 20 ng/mL followed by extraction with tert-butyl methyl ether and centrifugation. The organic layer was evaporated under N(2) gas and reconstituted with 100 μL of acetate buffer (pH 3.4, 20mM), and 50-μL portions of reconstituted sample were injected onto the column. Sample analysis including sample pretreatment was achieved within 35 min. Calibration curve was linear (r ≥ 0.998) from 5 to 100 ng/mL. Both intra- and inter-day assay precisions that are presented through RSD were lower than 12.6% for intra-day and lower than 12.0% for inter-day assessment. Limit of detection was 0.8 ng/mL at S/N of 3. This method was omitting the use of expensive solid phase extraction and time consuming liquid extraction procedures. Moreover, the present method was successfully applied to study pharmacokinetic parameters of FEN after intraperitoneal administration to male Wistar rat. Pharmacokinetic parameters estimated by using moment analysis were T(1/2) 198.3 ± 44.7 min, T(max) 28.3 ± 2.9 min and AUC(0-180) 15.6 ± 2.9(× 10(2))ngmin/mL. | |||||
書誌情報 |
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 巻 879, 号 27, p. 2941-2944, 発行日 2011-10-01 |
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出版者 | ||||||
出版者 | Elsevier BV | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 15700232 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1873376X | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11656178 | |||||
PubMed番号 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | PMID | |||||
関連識別子 | 21917532 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.jchromb.2011.08.029 | |||||
権利 | ||||||
権利情報 | Copyright © 2011 Elsevier B.V. All rights reserved. | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 879(27), pp.2941-2944; 2011 |