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Persistence and Dynamics of DNA Damage Signal Amplification Determined by Microcolony Formation and Live-cell Imaging
http://hdl.handle.net/10069/26955
http://hdl.handle.net/10069/26955712dc84f-170e-47ba-9a73-b9335a9d3e0a
名前 / ファイル | ライセンス | アクション |
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JRR52_766.pdf (889.4 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2011-12-27 | |||||
タイトル | ||||||
タイトル | Persistence and Dynamics of DNA Damage Signal Amplification Determined by Microcolony Formation and Live-cell Imaging | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | DNA damage foci | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | DNA damage response | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Live-cell imaging | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Oka, Yasuyoshi
× Oka, Yasuyoshi× Yamauchi, Motohiro× Suzuki, Masatoshi× Yamashita, Shunichi× Suzuki, Keiji |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Cell cycle checkpoints are essential cellular process protecting the integrity of the genome from DNA damaging agents. In the present study, we developed a microcolony assay, in which normal human diploid fibroblast-like cells exposed to ionizing radiation, were plated onto coverslips at very low density (3 cells/cm2). Cells were grown for up to 3 days, and phosphorylated ATM at Ser1981 and 53BP1 foci were analyzed as the markers for an amplified DNA damage signal. We observed a dose-dependent increase in the fraction of non-dividing cells, whose increase was compromised by knocking down p53 expression. While large persistent foci were predominantly formed in non-dividing cells, we observed some growing colonies that contained cells with large foci. As each microcolony was derived from a single cell, it appeared that some cells could proliferate with large foci. A live-imaging analysis using hTERT-immortalized normal human diploid cells transfected with the EGFP-tagged 53BP1 gene revealed that the formation of persistent large foci was highly dynamic. Delayed appearance and disappearance of large foci were frequently observed in exposed cells visualized 12–72 hours after X-irradiation. Thus, our results indicate that amplified DNA damage signal could be ignored, which may be explained in part by the dynamic nature of the amplification process. | |||||
書誌情報 |
Journal of Radiation Research 巻 52, 号 6, p. 766-774, 発行日 2011-11-22 |
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出版者 | ||||||
出版者 | Japan Radiation Research Society | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 04493060 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13499157 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00705792 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1269/jrr.10164 | |||||
権利 | ||||||
権利情報 | Copyright (c) 2011 by Journal of Radiation Research Editorial Committee | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Journal of Radiation Research, 52(6), pp.766-774; 2011 |