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In vivo and in vitro gametocyte production of Plasmodium falciparum isolates from Northern Thailand.
http://hdl.handle.net/10069/25120
http://hdl.handle.net/10069/251205abe6d9f-cf7d-4af4-8cd3-cd9ab561b79b
名前 / ファイル | ライセンス | アクション |
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IJP41_317.pdf (209.1 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2011-05-23 | |||||
タイトル | ||||||
タイトル | In vivo and in vitro gametocyte production of Plasmodium falciparum isolates from Northern Thailand. | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Allelic types | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Gametocyte detection | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Malaria | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Pfg377 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Plasmodium falciparum | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Nakazawa, Shusuke
× Nakazawa, Shusuke× Culleton, Richard× Maeno, Yoshimasa |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Understanding why some malaria-infected individuals are infective to mosquitoes while others are not, is of great importance when considering interventions to stop malaria transmission. Whether gametocytes are produced in every individual infected with Plasmodium falciparum remains unclear. Using a highly sensitive reverse transcription (RT)-PCR assay, we attempted to detect gametocyte-specific mRNA transcripts in isolates from Thai patients which newly adapted to continuous in vitro culture. We then compared the allelic types of the pfg377 gene between patient blood and culture-adapted parasites in order to determine whether the same parasite lines were producing gametocytes in vivo and in vitro. Transcripts of pfg377 were detected in all parasite isolates and in the corresponding cultured isolates, revealing that all patients had gametocytes circulating in their blood at the time of sampling. For isolates in continuous in vitro culture, there was a match between pfg377 allelic types detected by PCR from genomic DNA (and thus indicative of the dominant allelic type of asexual parasites) and those detected by RT-PCR of mRNA (gametocyte-specific), whereas in freshly isolated patient blood there were some differences between the asexual parasite allelic type and that of the gametocytes in the same infection. Seven isolates contained asexual stage parasites harbouring pfg377 alleles that were not detectable in gametocytes from the same infections, suggesting that some clones were not producing gametocytes at the time of sampling, or that they were below the level of detection. | |||||
書誌情報 |
International Journal for Parasitology 巻 41, 号 3-4, p. 317-323, 発行日 2011-03 |
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出版者 | ||||||
出版者 | Elsevier BV | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00207519 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 18790135 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA0068075X | |||||
PubMed番号 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | PMID | |||||
関連識別子 | 21110978 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.ijpara.2010.10.003 | |||||
権利 | ||||||
権利情報 | © 2010 Elsevier | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | International Journal for Parasitology, 41(3-4), pp.317-323; 2011 |