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Recombinant Porphyromonas gingivalis FimA preproprotein expressed in Escherichia coli is lipidated and the mature or processed recombinant FimA protein forms a short filament in vitro.
http://hdl.handle.net/10069/25139
http://hdl.handle.net/10069/25139261f358c-e0f8-4cca-b945-8303a38e3f0e
名前 / ファイル | ライセンス | アクション |
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CJM56_959.pdf (1.4 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2011-05-25 | |||||
タイトル | ||||||
タイトル | Recombinant Porphyromonas gingivalis FimA preproprotein expressed in Escherichia coli is lipidated and the mature or processed recombinant FimA protein forms a short filament in vitro. | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Fimbriae | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Lipoprotein | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Porphyromonas gingivalis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Recombinant FimA protein | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | TLR2 | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Shoji, Mikio
× Shoji, Mikio× Yoshimura, Atsutoshi× Yoshioka, Hidenobu× Takade, Akemi× Takuma, Yasuko× Yukitake, Hideharu× Naito, Mariko× Hara, Yoshitaka× Yoshida, Shin-Ichi× Nakayama, Koji |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The gram-negative anaerobic bacterium Porphyromonas gingivalis is an etiologically important pathogen for chronic periodontal diseases in adults. Our previous study suggested that the major structural components of both Fim and Mfa fimbriae in this organism are secreted through their lipidated precursors. In this study, we constructed Escherichia coli strains expressing various fimA genes with or without the 5'-terminal DNA region encoding the signal peptide, and we determined whether lipidation of recombinant FimA proteins occurred in E. coli. Lipidation occurred for a recombinant protein from the fimA gene with the 5'-terminal DNA region encoding the signal peptide but not for a recombinant protein from the fimA gene without the signal-peptide-encoding region, as revealed by [3H]palmitic acid labeling experiments. A TLR2-dependent signaling response was induced by the recombinant protein from the fimA gene with the signal-peptide-encoding region but not by a recombinant protein from the fimA gene with the signal-peptide-encoding region that had a base substitution causing an amino acid substitution (C19A). Electron microscopic analysis revealed that recombinant FimA (A-47 - W-383) protein was autopolymerized to form filamentous structures of about 80 nm in length in vitro. The results suggest that FimA protein, a major subunit of Fim fimbriae, is transported to the outer membrane by the lipoprotein sorting system, and a mature or processed FimA protein on the outer membrane is autopolymerized to form Fim fimbriae. | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | This is a revised document of the article published in Canadian Journal of Microbiology, 56(11), pp.959-967; 2010. The contents of modification is published in Canadian Journal of Microbiology, 57(1), pp.68; 2011. It is available at http://dx.doi.org/10.1139/W10-114. | |||||
書誌情報 |
Canadian Journal of Microbiology 巻 56, 号 11, p. 959-967, 発行日 2010-11 |
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出版者 | ||||||
出版者 | Canadian Science Publishing | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00084166 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 14803275 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00597703 | |||||
PubMed番号 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | PMID | |||||
関連識別子 | 21076487 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1139/w10-084 | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Canadian Journal of Microbiology, 56(11), pp.959-967; 2010 |