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Role of Ezrin Phosphorylation in HIV-1 Replication
http://hdl.handle.net/10069/38601
http://hdl.handle.net/10069/386010e2df989-5490-441d-840e-25603c371271
名前 / ファイル | ライセンス | アクション |
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Fmicb9_1912.pdf (2.0 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2018-09-27 | |||||
タイトル | ||||||
タイトル | Role of Ezrin Phosphorylation in HIV-1 Replication | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Ezri | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | HIV-1 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Viral assembly | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Viral budding | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Viral entry | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Kamiyama, Haruka
× Kamiyama, Haruka× Izumida, Mai× Umemura, Yuria× Hayashi, Hideki× Matsuyama, Toshifumi× Kubo, Yoshinao |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Host-cell expression of the ezrin protein is required for CXCR4 (X4)-tropic HIV-1 infection. Ezrin function is regulated by phosphorylation at threonine-567. This study investigates the role of ezrin phosphorylation in HIV-1 infection and virion release. We analyzed the effects of ezrin mutations involving substitution of threonine-567 by alanine (EZ-TA), a constitutively inactive mutant, or by aspartic acid (EZ-TD), which mimics phosphorylated threonine. We also investigated the effects of ezrin silencing on HIV-1 virion release using a specific siRNA. We observed that X4-tropic HIV-1 vector infection was inhibited by expression of the EZ-TA mutant but increased by expression of the EZ-TD mutant, suggesting that ezrin phosphorylation in target cells is required for efficient HIV-1 entry. Expression of a dominant-negative mutant of ezrin (EZ-N) and ezrin silencing in HIV-1 vector-producing cells significantly reduced the infectivity of released virions without affecting virion production. This result indicates that endogenous ezrin expression is required for virion infectivity. The EZ-TD but not the EZ-TA inhibited virion release from HIV-1 vector-producing cells. Taken together, these findings suggest that ezrin phosphorylation in target cells is required for efficient HIV-1 entry but inhibits virion release from HIV-1 vector-producing cells. |
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書誌情報 |
Frontiers in Microbiology 巻 9, p. 1912, 発行日 2018-08-27 |
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出版者 | ||||||
出版者 | Frontiers Media S.A. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1664302X | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1664-302X | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.3389/fmicb.2018.01912 | |||||
権利 | ||||||
権利情報 | c2018 Kamiyama, Izumida, Umemura, Hayashi, Matsuyama and Kubo. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Frontiers in Microbiology, 9, art.no.1912; 2018 |