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Creating localized DNA double-strand breaks with microirradiation.
http://hdl.handle.net/10069/25685
http://hdl.handle.net/10069/25685ffd22d64-910d-40dd-8013-367aaebedf98
名前 / ファイル | ライセンス | アクション |
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NatPro6_134.pdf (58.5 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2011-09-06 | |||||
タイトル | ||||||
タイトル | Creating localized DNA double-strand breaks with microirradiation. | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | DNA double strand breaks | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | micro-irradiation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | DNA damage-induced foci | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | local damage | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | DNA damage response | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | DNA repair | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Suzuki, Keiji
× Suzuki, Keiji× Yamauchi, Motohiro× Oka, Yasuyoshi× Suzuki, Masatoshi× Yamashita, Shunichi |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | We describe a protocol for creating localized DNA double-strand breaks (DSBs) with minimal requirements that can be applied in cell biology and molecular biology. This protocol is based on the combination of 5-bromo-2'-deoxyuridine (BrdU) labeling and ultraviolet C (UVC) irradiation through porous membranes. Cells are labeled with 10 μM BrdU for 48-72 h, washed with Ca(2+)- and Mg(2+)-free PBS(-), covered by polycarbonate membranes with micropores and exposed to UVC light. With this protocol, localized DSBs are created within subnuclear areas, irrespective of the cell cycle phase. Recruitment of proteins involved in DNA repair, DNA damage response, chromatin remodeling and histone modifications can be visualized without any specialized equipment. The quality is the same as that obtained by laser microirradiation or by any other focal irradiation. DSBs become visible within 30 min of UVC irradiation. | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | without figures | |||||
書誌情報 |
Nature Protocols 巻 6, 号 2, p. 134-139, 発行日 2011-02 |
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出版者 | ||||||
出版者 | Nature Publishing Group | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 17542189 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 17502799 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA1223996X | |||||
PubMed番号 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | PMID | |||||
関連識別子 | 21293454 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1038/nprot.2010.183 | |||||
権利 | ||||||
権利情報 | © 2011 Nature America, Inc. All rights reserved. | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Nature Protocols, 6(2), pp.134-139; 2011 |