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Analysis of the Cell Type-Dependence on the Arenavirus Z-Mediated Virus-Like Particle Production
http://hdl.handle.net/10069/40372
http://hdl.handle.net/10069/40372d06e030b-a316-495e-9817-73c08f91efbc
名前 / ファイル | ライセンス | アクション |
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Fmicb11_562814.pdf (3.9 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2020-10-30 | |||||
タイトル | ||||||
タイトル | Analysis of the Cell Type-Dependence on the Arenavirus Z-Mediated Virus-Like Particle Production | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | arenavirus | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | cell type-dependence | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | L-domain | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | virus-like particle | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Z | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Mpingabo, Patrick I.
× Mpingabo, Patrick I.× Urata, Shuzo× Yasuda, Jiro |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Several arenaviruses are highly pathogenic to humans, causing hemorrhagic fever. Discovery of anti-arenavirus drug candidates is urgently needed, although the molecular basis of the host- and organ-specific pathogenicity remains to be fully elucidated. The arenavirus Z protein facilitates production of virus-like particles (VLPs), providing an established method to assess virus budding. In this study, we examined the efficiency of VLP production by solely expressing Z protein of several different arenaviruses. In addition, we analyzed the role of the late (L)-domain of the arenavirus Z protein, which is essential for the interaction with ESCRT proteins, in VLP production among different cell lines. VLP assay was performed using Z proteins of Junin virus (JUNV), Machupo virus (MACV), Tacaribe virus (TCRV), Latino virus (LATV), Pichinde virus (PICV), and Lassa virus (LASV) in six different cell lines: HEK293T, Huh-7, A549, Vero76, BHK-21, and NIH3T3 cells. JUNV, MACV, and LASV Z proteins efficiently produced VLPs in all tested cell lines, while the efficiencies of VLP production by the other arenavirus Z proteins were cell type-dependent. The contribution of the L-domain(s) within Z protein to VLP production also highly depended on the cell type. These results suggested that each arenavirus has its own particle-production mechanism, which is different among the cell types. |
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書誌情報 |
Frontiers in Microbiology 巻 11, p. 562814, 発行日 2020-09-30 |
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出版者 | ||||||
出版者 | Frontiers Media S.A. | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1664302X | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.3389/fmicb.2020.562814 | |||||
権利 | ||||||
権利情報 | c 2020 Mpingabo, Urata and Yasuda. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Frontiers in Microbiology, 11, art.no.562814; 2020 |