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Axenic culture of Brachionus plicatilis using antibiotics
http://hdl.handle.net/10069/24463
http://hdl.handle.net/10069/244636b8cf5ac-8412-41eb-b522-ecee0283e626
名前 / ファイル | ライセンス | アクション |
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Hydrobiologia_Suga.pdf (527.6 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2010-11-09 | |||||
タイトル | ||||||
タイトル | Axenic culture of Brachionus plicatilis using antibiotics | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Antibiotic | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Bacteria free | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Brachionus plicatilis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Resting egg disinfection | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Suga, Koushirou
× Suga, Koushirou× Tanaka, Yukari× Sakakura, Yoshitaka× Hagiwara, Atsushi |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The rotifer Brachionus plicatilis culture is composed of complex microcosms including bacteria, protozoans, algae, and fungi. Previous studies reported methods to establish axenic rotifer cultures, but further refinement of these techniques is needed, for molecular biological research which requires pure culture to isolate nucleic acids from rotifers only. In order to render rotifer culture axenic, we tested five antibiotics: ampicillin (Amp), chloramphenicol (Cp), kanamycin (Km), nalidixic acid (Na), and streptomycin (Sm) at 30-100 μg/ml. Except for Cp, which reduces rotifer reproduction, all other antibiotics at the tested concentrations did not affect rotifer reproduction or show any toxic effects. A rotifer disinfection method was finally established by treating the resting eggs with 0.25% (w/v) sodium hypochlorite (NaOCl) for 3 min, washing with sterilized sea water, and then exposing the neonates to an Amp, Km, Na, and Sm mixture. Using four nutrient media, we confirmed that this protocol renders the rotifer culture bacterial and fungus free. The axenic rotifer culture generated here is useful not only for genetic analysis of Brachionus plicatilis, but for studying the rotifer life cycle without bacterial influence. | |||||
書誌情報 |
Hydrobiologia 巻 662, 号 1, p. 113-119, 発行日 2011-03 |
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出版者 | ||||||
出版者 | Springer | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00188158 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 15735117 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00666453 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1007/s10750-010-0488-0 | |||||
権利 | ||||||
権利情報 | © Springer Science+Business Media B.V. 2010 | |||||
権利 | ||||||
権利情報 | The original publication is available at www.springerlink.com | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Hydrobiologia, 662(1), pp.113-119; 2011 |