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These two differ in their promoter, but the amino acid sequences of the mature proteins are identical. In this study, we examined the relationship between the two types of TNSALP expression and osteoblast differentiation. Design: Gene expression of the two types of TNSALP was observed by reverse transcription-polymerase chain reaction. MC3T3-NM4 was sub-cloned from an established mouse osteoblastic cell line in which osteoblast characters do not appear without dexamethasone. The C2C12 mouse myoblastic cell line, which can be induced to osteoblasts with bone morphogenic protein 2, and organ-cultured tooth germs were also used in this work. Results: The gene expression of liver-type TNSALP was observed in only MC3T3-NM4 activated by dexamethasone. 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Liver-type of tissue non-specific alkaline phosphatase is induced during mouse bone and tooth cell differentiation
http://hdl.handle.net/10069/38712
http://hdl.handle.net/10069/387125acb10ef-6180-4bff-aa52-e787bbbc41ee
名前 / ファイル | ライセンス | アクション |
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AOB98_32.pdf (286.4 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2020-03-01 | |||||
タイトル | ||||||
タイトル | Liver-type of tissue non-specific alkaline phosphatase is induced during mouse bone and tooth cell differentiation | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Bone-type TNSALP | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Liver-type TNSALP | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Osteogenesis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Tissue non-specific alkaline phosphatase | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Tooth differentiation | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Baba, Tomomi T.
× Baba, Tomomi T.× Terashima, Tatsuo× Oida, Shinichiro |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Background and objective: Tissue non-specific alkaline phosphatase (TNSALP) contains two types?bone- and liver-type?which are produced from the same gene due to differences in splicing. These two differ in their promoter, but the amino acid sequences of the mature proteins are identical. In this study, we examined the relationship between the two types of TNSALP expression and osteoblast differentiation. Design: Gene expression of the two types of TNSALP was observed by reverse transcription-polymerase chain reaction. MC3T3-NM4 was sub-cloned from an established mouse osteoblastic cell line in which osteoblast characters do not appear without dexamethasone. The C2C12 mouse myoblastic cell line, which can be induced to osteoblasts with bone morphogenic protein 2, and organ-cultured tooth germs were also used in this work. Results: The gene expression of liver-type TNSALP was observed in only MC3T3-NM4 activated by dexamethasone. For C2C12, the gene expression of bone-type TNSALP was observed even in non-induced conditions where myotubes were formed, whereas the liver-type TNSALP mRNA was only expressed when C2C12 differentiated into osteoblasts by bone morphogenic protein 2. Furthermore, in the organ-cultured tooth germs, the liver-type TNSALP mRNA was expressed according to differentiation of tooth germs. Conclusion: These results suggest that the liver-type TNSALP mRNA is induced according to differentiation of bone and tooth. | |||||
書誌情報 |
Archives of Oral Biology 巻 98, p. 32-37, 発行日 2019-02 |
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出版者 | ||||||
出版者 | Elsevier Ltd | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00039969 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.archoralbio.2018.10.036 | |||||
権利 | ||||||
権利情報 | c2018 Elsevier Ltd. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Archives of Oral Biology, 98, pp.32-37; 2019 |