WEKO3
アイテム
{"_buckets": {"deposit": "3cd46b29-4718-4698-be9f-ebaeab0b7bb3"}, "_deposit": {"created_by": 2, "id": "1470", "owners": [2], "pid": {"revision_id": 0, "type": "depid", "value": "1470"}, "status": "published"}, "_oai": {"id": "oai:nagasaki-u.repo.nii.ac.jp:00001470", "sets": ["36"]}, "author_link": ["7016", "7017"], "item_2_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2018-09-30", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "5", "bibliographicPageEnd": "387", "bibliographicPageStart": "382", "bibliographicVolumeNumber": "71", "bibliographic_titles": [{"bibliographic_title": "Japanese Journal of Infectious Diseases"}]}]}, "item_2_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "The possible emergence of praziquantel (PZQ)-tolerant and/or-resistant schistosomes requires the study and development of new antischistosomal drugs as alternatives to PZQ. The present study investigates the capability of 3 dyes?methylene blue (MB), neutral red (NR), and trypan blue (TB)?to assess the in vitro antischistosomal effect of antimalarial drugs on Schistosoma mansoni adult worms. S. mansoni adult worms were incubated in the medium alone as the control or in the medium supplemented with 10 μg/ml primaquine (PQ), artesunate (AR), or amodiaquine (AQ) for 5 days. Viabili-ties of the worms were observed following staining with MB, NR, or TB. The disparity of MB and NR staining among male and female adult worms treated with PQ, AR, and AQ correlated with the various levels of damage to the male and female worms.Furthermore, the severity of the damage to the adult worms treated with the 3 drugs appeared to be reflected in the TB staining status. \nThe results indicate that the 3 non-fluorescent dyes can serve as useful complementary tools to assess the antischistosomal effect of antimalarial drugs.", "subitem_description_type": "Abstract"}]}, "item_2_description_63": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "Japanese Journal of Infectious Diseases, 71(5), pp.382-387; 2018", "subitem_description_type": "Other"}]}, "item_2_publisher_33": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "国立感染症研究所"}]}, "item_2_relation_12": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "10.7883/yoken.JJID.2018.034", "subitem_relation_type_select": "DOI"}}]}, "item_2_rights_13": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "c 2018 National Institute of Infectious Diseases"}]}, "item_2_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "13446304", "subitem_source_identifier_type": "ISSN"}]}, "item_2_source_id_8": {"attribute_name": "EISSN", "attribute_value_mlt": [{"subitem_source_identifier": "18842836", "subitem_source_identifier_type": "ISSN"}]}, "item_2_version_type_16": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Mitsui, Yoshinori"}], "nameIdentifiers": [{"nameIdentifier": "7016", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kato, Kentaro"}], "nameIdentifiers": [{"nameIdentifier": "7017", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-12-18"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "JJID71_382.pdf", "filesize": [{"value": "2.0 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 2000000.0, "url": {"label": "JJID71_382.pdf", "url": "https://nagasaki-u.repo.nii.ac.jp/record/1470/files/JJID71_382.pdf"}, "version_id": "9a88865c-22f4-4527-8b9c-050a3b0ada67"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "methylene blue", "subitem_subject_scheme": "Other"}, {"subitem_subject": "neutral red", "subitem_subject_scheme": "Other"}, {"subitem_subject": "trypan blue", "subitem_subject_scheme": "Other"}, {"subitem_subject": "antimalarial drug", "subitem_subject_scheme": "Other"}, {"subitem_subject": "antischistosomal effect", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Application of Non-Fluorescent Dyes to Assess the Antischistosomal Effect of Antimalarial Drugs on Schistosoma mansoni Adult Worms", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Application of Non-Fluorescent Dyes to Assess the Antischistosomal Effect of Antimalarial Drugs on Schistosoma mansoni Adult Worms"}]}, "item_type_id": "2", "owner": "2", "path": ["36"], "permalink_uri": "http://hdl.handle.net/10069/38735", "pubdate": {"attribute_name": "公開日", "attribute_value": "2018-12-18"}, "publish_date": "2018-12-18", "publish_status": "0", "recid": "1470", "relation": {}, "relation_version_is_last": true, "title": ["Application of Non-Fluorescent Dyes to Assess the Antischistosomal Effect of Antimalarial Drugs on Schistosoma mansoni Adult Worms"], "weko_shared_id": -1}
Application of Non-Fluorescent Dyes to Assess the Antischistosomal Effect of Antimalarial Drugs on Schistosoma mansoni Adult Worms
http://hdl.handle.net/10069/38735
http://hdl.handle.net/10069/3873527e81b79-803b-4f5d-8fb9-d67c27846d9e
名前 / ファイル | ライセンス | アクション |
---|---|---|
JJID71_382.pdf (2.0 MB)
|
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2018-12-18 | |||||
タイトル | ||||||
タイトル | Application of Non-Fluorescent Dyes to Assess the Antischistosomal Effect of Antimalarial Drugs on Schistosoma mansoni Adult Worms | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | methylene blue | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | neutral red | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | trypan blue | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | antimalarial drug | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | antischistosomal effect | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Mitsui, Yoshinori
× Mitsui, Yoshinori× Kato, Kentaro |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The possible emergence of praziquantel (PZQ)-tolerant and/or-resistant schistosomes requires the study and development of new antischistosomal drugs as alternatives to PZQ. The present study investigates the capability of 3 dyes?methylene blue (MB), neutral red (NR), and trypan blue (TB)?to assess the in vitro antischistosomal effect of antimalarial drugs on Schistosoma mansoni adult worms. S. mansoni adult worms were incubated in the medium alone as the control or in the medium supplemented with 10 μg/ml primaquine (PQ), artesunate (AR), or amodiaquine (AQ) for 5 days. Viabili-ties of the worms were observed following staining with MB, NR, or TB. The disparity of MB and NR staining among male and female adult worms treated with PQ, AR, and AQ correlated with the various levels of damage to the male and female worms.Furthermore, the severity of the damage to the adult worms treated with the 3 drugs appeared to be reflected in the TB staining status. The results indicate that the 3 non-fluorescent dyes can serve as useful complementary tools to assess the antischistosomal effect of antimalarial drugs. |
|||||
書誌情報 |
Japanese Journal of Infectious Diseases 巻 71, 号 5, p. 382-387, 発行日 2018-09-30 |
|||||
出版者 | ||||||
出版者 | 国立感染症研究所 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13446304 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 18842836 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.7883/yoken.JJID.2018.034 | |||||
権利 | ||||||
権利情報 | c 2018 National Institute of Infectious Diseases | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Japanese Journal of Infectious Diseases, 71(5), pp.382-387; 2018 |