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Comparative study on modeccin- and phytohemagglutinin (PHA)-induced secretion of cytokines and nitric oxide (NO) in RAW264.7 cells.
http://hdl.handle.net/10069/27359
http://hdl.handle.net/10069/2735978064959-4abd-4bf5-80ad-e121d3b3a6c5
名前 / ファイル | ライセンス | アクション |
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ABBS43_52.pdf (265.2 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2012-02-01 | |||||
タイトル | ||||||
タイトル | Comparative study on modeccin- and phytohemagglutinin (PHA)-induced secretion of cytokines and nitric oxide (NO) in RAW264.7 cells. | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | G-CSF | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | modeccin | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | nitric oxide | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | PHA | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | TNF- | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Kim, Daekyung
× Kim, Daekyung× Yamasaki, Yasuhiro× Jiang, Zedong× Nakayama, Yuki× Yamanishi, Tomohiro× Yamaguchi, Kenichi× Oda, Tatsuya |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The effects of cytotoxic lectins, modeccin and phytohemagglutinin (PHA) on mouse macrophage cell line RAW264.7 was studied by detecting the induction of inflammatory mediators. Results showed that modeccin induced the release of tumor necrosis factor-α (TNF-α) from RAW264.7 cells with a bell-shape concentration-dependent profile. PHA that showed no significant cytotoxicity on RAW264.7 cells up to 100,000 ng/ml induced much higher level of TNF-α than modeccin. PHA simultaneously induced the secretion of granulocyte colony stimulation factor (G-CSF) from RAW264.7 cells with even much higher level than that of TNF-α, whereas modeccin did not. Furthermore, PHA induced the secretion of nitric oxide (NO) in RAW264.7 cells, while no significant level of NO was detected in the modeccin-treated cells. NH₄Cl (a lysomotoropic agent) and cycloheximide (a ribosome inhibitor) strongly inhibited modeccin-induced TNF-α secretion, but no significant inhibitory effects of these reagents on the PHA-induced TNF-α secretion were observed. Contrary to modeccin-induced TNF-α secretion, even slightly increased TNF-α secretion was observed in PHA-treated cells in the presence of 10 mM NH₄Cl. In addition, the inhibition profiles of modeccin-induced TNF-α secretion by various kinase inhibitors were different from those of PHA. These results suggested that the action mode of modeccin to stimulate RAW264.7 cells leading to the secretion of inflammatory molecules, including TNF-α, is distinct from that of PHA. On the other hand, significantly increased translocation of activator protein-1 (AP-1), a crucial transcription factor involved in expression of inflammatory molecules, into nucleus was observed in RAW264.7 cells treated with PHA and modeccin. | |||||
書誌情報 |
Acta biochimica et biophysica Sinica 巻 43, 号 1, p. 52-60, 発行日 2011-01 |
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出版者 | ||||||
出版者 | Institute of Biochemistry and Cell Biology | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 17457270 | |||||
PubMed番号 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | PMID | |||||
関連識別子 | 21148191 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1093/abbs/gmq105 | |||||
権利 | ||||||
権利情報 | © The Author 2010. Published by ABBS Editorial Office in association with Oxford University Press on behalf of the Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences. | |||||
権利 | ||||||
権利情報 | This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Acta biochimica et biophysica Sinica following peer review. The definitive publisher-authenticated version Acta biochimica et biophysica Sinica, 43(1), pp.52-60; 2011 is available online at: http://abbs.oxfordjournals.org/content/43/1/52. | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Acta biochimica et biophysica Sinica, 43(1), pp.52-60; 2011 |