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Selective determination of ubiquinone in human plasma by HPLC with chemiluminescence reaction based on the redox cycle of quinone.
http://hdl.handle.net/10069/24838
http://hdl.handle.net/10069/248386de7affe-981d-4435-8536-434f61d0e8fa
名前 / ファイル | ライセンス | アクション |
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ABC400_381.pdf (159.4 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2011-04-01 | |||||
タイトル | ||||||
タイトル | Selective determination of ubiquinone in human plasma by HPLC with chemiluminescence reaction based on the redox cycle of quinone. | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Chemiluminescence | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | HPLC | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Human plasma | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Redox cycle | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Ubiquinone | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Kishikawa, Naoya
× Kishikawa, Naoya× Ohkubo, Nobuhiro× Ohyama, Kaname× Nakashima, Kenichiro× Kuroda, Naotaka |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Ubiquinone is an important biologically active compound in the living body. The determination of ubiquinone in human plasma is useful for the investigation of bioavailability of ubiquinone and for early diagnosis of several diseases. Therefore, we developed a high-performance liquid chromatography (HPLC) with chemiluminescence detection method for the analysis of ubiquinone in plasma samples. The method is based on luminol chemiluminescence detection of super oxide anion that is generated by the redox cycle reaction between ubiquinone and dithiothreitol. The HPLC system involved an octyl column with a mobile phase of methanol. Ubiquinone eluted from the column was mixed with dithiothreitol and luminol solutions simultaneously, and generated chemiluminescence was monitored by chemiluminescence detector. The calibration curve for standard ubiquinone solution was linear from 0.09 to 43.2 μg/mL (0.45-216 ng on column) with the correlation coefficient of 0.999, and the detection limit (S/N = 3) was 26 ng/mL (130 pg on column). Using the proposed HPLC method, the peak of ubiquinone in human plasma could be clearly detected on the chromatogram without any interference from plasma components. | |||||
書誌情報 |
Analytical and bioanalytical chemistry 巻 400, 号 2, p. 381-385, 発行日 2011-04 |
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出版者 | ||||||
出版者 | Springer Verlag | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 16182642 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 16182650 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11610734 | |||||
PubMed番号 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | PMID | |||||
関連識別子 | 21246189 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1007/s00216-011-4662-7 | |||||
権利 | ||||||
権利情報 | © 2011 Springer-Verlag. | |||||
権利 | ||||||
権利情報 | The original publication is available at www.springerlink.com | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Analytical and bioanalytical chemistry, 400(2), pp.381-385; 2011 |