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Ultrasensitive human prion detection in cerebrospinal fluid by real-time quaking-induced conversion.
http://hdl.handle.net/10069/25642
http://hdl.handle.net/10069/2564244b73d60-3820-40ca-a4e4-ae540a78fb68
名前 / ファイル | ライセンス | アクション |
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NatMEd17_175.pdf (1.1 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2011-09-01 | |||||
タイトル | ||||||
タイトル | Ultrasensitive human prion detection in cerebrospinal fluid by real-time quaking-induced conversion. | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Atarashi, Ryuichiro
× Atarashi, Ryuichiro× Satoh, Katsuya× Sano, Kazunori× Fuse, Takayuki× Yamaguchi, Naohiro× Ishibashi, Daisuke× Matsubara, Takehiro× Nakagaki, Takehiro× Yamanaka, Hitoki× Shirabe, Susumu× Yamada, Masahito× Mizusawa, Hidehiro× Kitamoto, Tetsuyuki× Klug, Genevieve× McGlade, Amelia× Collins, Steven J× Nishida, Noriyuki |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The development of technologies for the in vitro amplification of abnormal conformations of prion protein (PrP(Sc)) has generated the potential for sensitive detection of prions. Here we developed a new PrP(Sc) amplification assay, called real-time quaking-induced conversion (RT-QUIC), which allows the detection of ≥1 fg of PrP(Sc) in diluted Creutzfeldt-Jakob disease (CJD) brain homogenate. Moreover, we assessed the technique first in a series of Japanese subjects and then in a blind study of 30 cerebrospinal fluid specimens from Australia, which achieved greater than 80% sensitivity and 100% specificity. These findings indicate the promising enhanced diagnostic capacity of RT-QUIC in the antemortem evaluation of suspected CJD. | |||||
書誌情報 |
Nature Medicine 巻 17, 号 2, p. 175-178, 発行日 2011-02 |
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出版者 | ||||||
出版者 | Nature Publishing Group | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 10788956 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1546170X | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11029600 | |||||
PubMed番号 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | PMID | |||||
関連識別子 | 21278748 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1038/nm.2294 | |||||
権利 | ||||||
権利情報 | © 2011 Nature America, Inc. All rights reserved. | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Nature Medicine, 17(2), pp.175-178; 2011 |