WEKO3
アイテム
{"_buckets": {"deposit": "b35662cb-8520-4a75-9b41-f01422828fbf"}, "_deposit": {"created_by": 2, "id": "15191", "owners": [2], "pid": {"revision_id": 0, "type": "depid", "value": "15191"}, "status": "published"}, "_oai": {"id": "oai:nagasaki-u.repo.nii.ac.jp:00015191", "sets": ["26"]}, "author_link": ["55859", "55862", "55861", "55860"], "item_2_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2010-05-04", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "5", "bibliographicPageEnd": "516", "bibliographicPageStart": "506", "bibliographicVolumeNumber": "9", "bibliographic_titles": [{"bibliographic_title": "DNA repair"}]}]}, "item_2_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Nucleotide excision repair (NER) removes the major UV-photolesions from cellular DNA. In humans, compromised NER activity is the cause of several photosensitive diseases, one of which is the skin-cancer predisposition disorder, xeroderma pigmentosum (XP). Two assays commonly used in measurement of NER activity are \u0027unscheduled DNA synthesis (UDS)\u0027, and \u0027recovery of RNA synthesis (RRS)\u0027, the latter being a specific measure of the transcription-coupled repair sub-pathway of NER. Both assays are key techniques for research in NER as well as in diagnoses of NER-related disorders. Until very recently, reliable methods for these assays involved measurements of incorporation of radio-labeled nucleosides. We have established non-radioactive procedures for determining UDS and RRS levels by incorporation of recently developed alkyne-conjugated nucleoside analogues, 5-ethynyl-2\u0027-deoxyuridine (EdU) and 5-ethynyuridine (EU). EdU and EU are respectively used as alternatives for (3)H-thymidine in UDS and for (3)H-uridine in RRS. Based on these alkyne-nucleosides and an integrated image analyser, we have developed a semi-automated assay system for NER-activity. We demonstrate the utility of this system for NER-activity assessments of lymphoblastoid samples as well as primary fibroblasts. Potential use of the system for large-scale siRNA-screening for novel NER defects as well as for routine XP diagnosis are also considered.", "subitem_description_type": "Abstract"}]}, "item_2_description_63": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "DNA repair, 9(5), pp.506-516; 2010", "subitem_description_type": "Other"}]}, "item_2_publisher_33": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Elsevier B.V."}]}, "item_2_relation_11": {"attribute_name": "PubMed番号", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "20171149", "subitem_relation_type_select": "PMID"}}]}, "item_2_relation_12": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1016/j.dnarep.2010.01.015", "subitem_relation_type_select": "DOI"}}]}, "item_2_rights_13": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "Copyright © 2010 Elsevier B.V. All rights reserved."}]}, "item_2_source_id_10": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA11032547", "subitem_source_identifier_type": "NCID"}]}, "item_2_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "15687864", "subitem_source_identifier_type": "ISSN"}]}, "item_2_source_id_8": {"attribute_name": "EISSN", "attribute_value_mlt": [{"subitem_source_identifier": "15687856", "subitem_source_identifier_type": "ISSN"}]}, "item_2_version_type_16": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Nakazawa, Yuka"}], "nameIdentifiers": [{"nameIdentifier": "55859", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yamashita, Shunichi"}], "nameIdentifiers": [{"nameIdentifier": "55860", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Lehmann, Alan R"}], "nameIdentifiers": [{"nameIdentifier": "55861", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ogi, Tomoo"}], "nameIdentifiers": [{"nameIdentifier": "55862", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-12-23"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "DNA_Ogi.pdf", "filesize": [{"value": "534.7 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 534700.0, "url": {"label": "DNA_Ogi.pdf", "url": "https://nagasaki-u.repo.nii.ac.jp/record/15191/files/DNA_Ogi.pdf"}, "version_id": "66ddbbe2-e848-4f07-a3b7-5e6c25c302ce"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Cockayne syndrome (CS)", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Nucleotide excision repair (NER)", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Recovery of RNA synthesis (RRS)", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Transcription-coupled repair (TCR)", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Unscheduled DNA synthesis (UDS)", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Xeroderma pigmentosum (XP)", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "A semi-automated non-radioactive system for measuring recovery of RNA synthesis and unscheduled DNA synthesis using ethynyluracil derivatives.", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "A semi-automated non-radioactive system for measuring recovery of RNA synthesis and unscheduled DNA synthesis using ethynyluracil derivatives."}]}, "item_type_id": "2", "owner": "2", "path": ["26"], "permalink_uri": "http://hdl.handle.net/10069/23067", "pubdate": {"attribute_name": "公開日", "attribute_value": "2010-03-15"}, "publish_date": "2010-03-15", "publish_status": "0", "recid": "15191", "relation": {}, "relation_version_is_last": true, "title": ["A semi-automated non-radioactive system for measuring recovery of RNA synthesis and unscheduled DNA synthesis using ethynyluracil derivatives."], "weko_shared_id": 2}
A semi-automated non-radioactive system for measuring recovery of RNA synthesis and unscheduled DNA synthesis using ethynyluracil derivatives.
http://hdl.handle.net/10069/23067
http://hdl.handle.net/10069/23067a38b6dca-37f4-41b9-879d-f52706f6baf2
名前 / ファイル | ライセンス | アクション |
---|---|---|
DNA_Ogi.pdf (534.7 kB)
|
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2010-03-15 | |||||
タイトル | ||||||
タイトル | A semi-automated non-radioactive system for measuring recovery of RNA synthesis and unscheduled DNA synthesis using ethynyluracil derivatives. | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Cockayne syndrome (CS) | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Nucleotide excision repair (NER) | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Recovery of RNA synthesis (RRS) | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Transcription-coupled repair (TCR) | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Unscheduled DNA synthesis (UDS) | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Xeroderma pigmentosum (XP) | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Nakazawa, Yuka
× Nakazawa, Yuka× Yamashita, Shunichi× Lehmann, Alan R× Ogi, Tomoo |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Nucleotide excision repair (NER) removes the major UV-photolesions from cellular DNA. In humans, compromised NER activity is the cause of several photosensitive diseases, one of which is the skin-cancer predisposition disorder, xeroderma pigmentosum (XP). Two assays commonly used in measurement of NER activity are 'unscheduled DNA synthesis (UDS)', and 'recovery of RNA synthesis (RRS)', the latter being a specific measure of the transcription-coupled repair sub-pathway of NER. Both assays are key techniques for research in NER as well as in diagnoses of NER-related disorders. Until very recently, reliable methods for these assays involved measurements of incorporation of radio-labeled nucleosides. We have established non-radioactive procedures for determining UDS and RRS levels by incorporation of recently developed alkyne-conjugated nucleoside analogues, 5-ethynyl-2'-deoxyuridine (EdU) and 5-ethynyuridine (EU). EdU and EU are respectively used as alternatives for (3)H-thymidine in UDS and for (3)H-uridine in RRS. Based on these alkyne-nucleosides and an integrated image analyser, we have developed a semi-automated assay system for NER-activity. We demonstrate the utility of this system for NER-activity assessments of lymphoblastoid samples as well as primary fibroblasts. Potential use of the system for large-scale siRNA-screening for novel NER defects as well as for routine XP diagnosis are also considered. | |||||
書誌情報 |
DNA repair 巻 9, 号 5, p. 506-516, 発行日 2010-05-04 |
|||||
出版者 | ||||||
出版者 | Elsevier B.V. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 15687864 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 15687856 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11032547 | |||||
PubMed番号 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | PMID | |||||
関連識別子 | 20171149 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.dnarep.2010.01.015 | |||||
権利 | ||||||
権利情報 | Copyright © 2010 Elsevier B.V. All rights reserved. | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | DNA repair, 9(5), pp.506-516; 2010 |