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Pirfenidone inhibits the expression of HSP47 in TGF-beta1-stimulated human lung fibroblasts.
http://hdl.handle.net/10069/23124
http://hdl.handle.net/10069/23124ed9c3df4-5726-48fd-9a21-a9fe86ff4bc6
名前 / ファイル | ライセンス | アクション |
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LifSci82_210.pdf (664.4 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2010-04-20 | |||||
タイトル | ||||||
タイトル | Pirfenidone inhibits the expression of HSP47 in TGF-beta1-stimulated human lung fibroblasts. | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Collagen type I | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Fibroblast | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Heat shock protein 47 (HSP47) | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Idiopathic pulmonary fibrosis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Lung fibrosis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Pirfenidone | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Nakayama, Seiko
× Nakayama, Seiko× Mukae, Hiroshi× Sakamoto, Noriho× Kakugawa, Tomoyuki× Yoshioka, Sumako× Soda, Hiroshi× Oku, Hisashi× Urata, Yoshie× Kondo, Takahito× Kubota, Hiroshi× Nagata, Kazuhiro× Kohno, Shigeru |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Pirfenidone (5-methyl-1-phenyl-2-(1H)-pyridone) is a novel anti-fibrotic and anti-inflammatory agent that inhibits the progression of fibrosis in animal models and patients with idiopathic pulmonary fibrosis (IPF). Heat shock protein (HSP) 47, a collagen-specific molecular chaperone, is involved in the processing and/or secretion of procollagen and plays an important role in the pathogenesis of IPF. The present study evaluated the in vitro effects of pirfenidone on expression of HSP47 and collagen type I in cultured normal human lung fibroblasts (NHLF). Expression levels of HSP47 and collagen type I in NHLF stimulated by transforming growth factor (TGF)-beta1 were evaluated genetically, immunologically and immunocytochemically. Treatment with TGF-beta1 stimulated both mRNA and protein expressions of both HSP47 and collagen type I in NHLF, and pirfenidone significantly inhibited this TGF-beta1-enhanced expression in a dose-dependent manner. We concluded that the anti-fibrotic effect of pirfenidone may be mediated not only through direct inhibition of collagen type I expression but also at least partly through inhibition of HSP47 expression in lung fibroblasts, with a resultant reduction of collagen synthesis in lung fibrosis. | |||||
書誌情報 |
Life sciences 巻 82, 号 3-4, p. 210-217, 発行日 2008-01-16 |
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出版者 | ||||||
出版者 | Elsevier Inc. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00243205 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00717011 | |||||
PubMed番号 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | PMID | |||||
関連識別子 | 18093617 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.lfs.2007.11.003 | |||||
権利 | ||||||
権利情報 | Copyright © 2007 Elsevier Inc. All rights reserved. | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Life sciences, 82(3-4), pp.210-217; 2008 |