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Automated analysis of the serum antioxidative activities against five different reactive oxygen species by sequential injection system with a chemiluminescence detector.
http://hdl.handle.net/10069/23181
http://hdl.handle.net/10069/2318141b301af-ddf0-433a-8122-9a14844f6a37
名前 / ファイル | ライセンス | アクション |
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CCA411_1111.pdf (164.9 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2010-06-01 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Automated analysis of the serum antioxidative activities against five different reactive oxygen species by sequential injection system with a chemiluminescence detector. | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Antioxidative activity | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Luminol chemiluminescence | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Reactive oxygen species | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Sequential injection analysis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Serum | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Kishikawa, Naoya
× Kishikawa, Naoya× Ohyama, Kaname× Yao, Junko× Miyamoto, Aoi× Imazato, Takahiro× Ueki, Yukitaka× Nakashima, Kenichiro× Maehata, Eisuke× Kuroda, Naotaka |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | BACKGROUND: There is a growing evidence that reactive oxygen species (ROS) may cause many pathologic conditions including chronic diseases, neurodegenerative disorders, cancer and aging. There are a number of methods to measure the total antioxidative activity of the serum. However, since the lifetime and oxidative activity of various types of ROS are all different, to measure simply the total antioxidative activity of the serum is not enough. Therefore, to aid the diagnosis and to improve the therapeutic strategy, it is important to develop a simple and reliable method of assaying antioxidative activity of the serum. METHODS: A method that combines sequential injection analysis (SIA) and luminol chemiluminescence (CL) detection was employed for the measurement of antioxidative activities of human serum. We collected sera from healthy subjects (n=42) and patients with diabetes (n=39) and rheumatoid arthritis (n=25) and tested the sensitivity, reproducibility and reliability of our method. RESULTS: Since the operation is automatically controlled by a personal computer, we obtained a satisfactory repeatability without the need of much manpower. The time required for obtaining the antioxidative activity against one ROS for one individual is less than 3min. Although the value of antioxidative activity varies from subject to subject, there were a certain relationship between the disease and the antioxidative values of each type of ROS. The results suggest that the measurement of antioxidative activity against different ROS may provide us with valuable information regarding the disease state. CONCLUSIONS: The evaluation of antioxidative activities against each ROS by the proposed method should be more informative to understand the antioxidative status of biological fluid. | |||||
書誌情報 |
Clinica chimica acta; international journal of clinical chemistry 巻 411, 号 15-16, p. 1111-1115, 発行日 2010-04-11 |
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出版者 | ||||||
出版者 | Elsevier B.V. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00098981 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 18733492 | |||||
書誌レコードID | ||||||
識別子タイプ | NCID | |||||
関連識別子 | AA11523593 | |||||
PubMed番号 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | PMID | |||||
関連識別子 | 20388503 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.cca.2010.04.005 | |||||
権利 | ||||||
権利情報 | Crown copyright © 2010 Published by Elsevier B.V. | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Clinica chimica acta, 411(15-16), pp.1111-1115; 2010 |