WEKO3
アイテム
{"_buckets": {"deposit": "5b384c41-8862-46c1-b673-9706f8bb9573"}, "_deposit": {"created_by": 2, "id": "15317", "owners": [2], "pid": {"revision_id": 0, "type": "depid", "value": "15317"}, "status": "published"}, "_oai": {"id": "oai:nagasaki-u.repo.nii.ac.jp:00015317", "sets": ["26"]}, "author_link": ["56640", "56642", "56639", "56641", "56638", "56644", "56643"], "item_2_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2010-05-28", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "2", "bibliographicPageEnd": "494", "bibliographicPageStart": "489", "bibliographicVolumeNumber": "396", "bibliographic_titles": [{"bibliographic_title": "Biochemical and biophysical research communications"}]}]}, "item_2_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "It is known that dehydroepiandrosterone (DHEA) inhibits a phenotypic switch in vascular smooth muscle cells (VSMC) induced by platelet-derived growth factor (PDGF)-BB. However, the mechanism behind the effect of DHEA on VSMC is not clear. Previously we reported that low molecular weight-protein tyrosine phosphatase (LMW-PTP) dephosphorylates PDGF receptor (PDGFR)-beta via a redox-dependent mechanism involving glutathione (GSH)/glutaredoxin (GRX)1. Here we demonstrate that the redox regulation of PDGFR-beta is involved in the effect of DHEA on VSMC. DHEA suppressed the PDGF-BB-dependent phosphorylation of PDGFR-beta. As expected, DHEA increased the levels of GSH and GRX1, and the GSH/GRX1 system maintained the redox state of LMW-PTP. Down-regulation of the expression of LMW-PTP using siRNA restored the suppression of PDGFR-beta-phosphorylation by DHEA. A promoter analysis of GRX1 and gamma-glutamylcysteine synthetase (gamma-GCS), a rate-limiting enzyme of GSH synthesis, showed that DHEA up-regulated the transcriptional activity at the peroxisome proliferator-activated receptor (PPAR) response element, suggesting PPARalpha plays a role in the induction of GRX1 and gamma-GCS expression by DHEA. In conclusion, the redox regulation of PDGFR-beta is involved in the suppressive effect of DHEA on VSMC proliferation through the up-regulation of GSH/GRX system.", "subitem_description_type": "Abstract"}]}, "item_2_description_63": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "Biochemical and biophysical research communications, 396(2), pp.489-494; 2010", "subitem_description_type": "Other"}]}, "item_2_publisher_33": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Elsevier Inc."}]}, "item_2_relation_11": {"attribute_name": "PubMed番号", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "20420805", "subitem_relation_type_select": "PMID"}}]}, "item_2_relation_12": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1016/j.bbrc.2010.04.125", "subitem_relation_type_select": "DOI"}}]}, "item_2_rights_13": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "Copyright © 2010 Elsevier Inc. All rights reserved."}]}, "item_2_source_id_10": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA00564395", "subitem_source_identifier_type": "NCID"}]}, "item_2_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0006291X", "subitem_source_identifier_type": "ISSN"}]}, "item_2_source_id_8": {"attribute_name": "EISSN", "attribute_value_mlt": [{"subitem_source_identifier": "10902104", "subitem_source_identifier_type": "ISSN"}]}, "item_2_version_type_16": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Urata, Yoshishige"}], "nameIdentifiers": [{"nameIdentifier": "56638", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Goto, Shinji"}], "nameIdentifiers": [{"nameIdentifier": "56639", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kawakatsu, Miho"}], "nameIdentifiers": [{"nameIdentifier": "56640", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yodoi, Junji"}], "nameIdentifiers": [{"nameIdentifier": "56641", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Eto, Masato"}], "nameIdentifiers": [{"nameIdentifier": "56642", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Akishita, Masahiro"}], "nameIdentifiers": [{"nameIdentifier": "56643", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kondo, Takahito"}], "nameIdentifiers": [{"nameIdentifier": "56644", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-12-23"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "BBRC396_489.pdf", "filesize": [{"value": "527.8 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 527800.0, "url": {"label": "BBRC396_489.pdf", "url": "https://nagasaki-u.repo.nii.ac.jp/record/15317/files/BBRC396_489.pdf"}, "version_id": "60b5e9fd-8645-46ac-b642-e81fe6eab0b1"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Dehydroepiandrosterone", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Glutaredoxin 1", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Glutathione", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Low molecular weight-protein tyrosine phosphatase", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Platelet-derived growth factor", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Vascular smooth muscle cells", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "DHEA attenuates PDGF-induced phenotypic proliferation of vascular smooth muscle A7r5 cells through redox regulation.", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "DHEA attenuates PDGF-induced phenotypic proliferation of vascular smooth muscle A7r5 cells through redox regulation."}]}, "item_type_id": "2", "owner": "2", "path": ["26"], "permalink_uri": "http://hdl.handle.net/10069/23191", "pubdate": {"attribute_name": "公開日", "attribute_value": "2010-06-09"}, "publish_date": "2010-06-09", "publish_status": "0", "recid": "15317", "relation": {}, "relation_version_is_last": true, "title": ["DHEA attenuates PDGF-induced phenotypic proliferation of vascular smooth muscle A7r5 cells through redox regulation."], "weko_shared_id": 2}
DHEA attenuates PDGF-induced phenotypic proliferation of vascular smooth muscle A7r5 cells through redox regulation.
http://hdl.handle.net/10069/23191
http://hdl.handle.net/10069/23191c84160e8-5b24-4ad8-8859-a5438a6e6b12
名前 / ファイル | ライセンス | アクション |
---|---|---|
BBRC396_489.pdf (527.8 kB)
|
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2010-06-09 | |||||
タイトル | ||||||
タイトル | DHEA attenuates PDGF-induced phenotypic proliferation of vascular smooth muscle A7r5 cells through redox regulation. | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Dehydroepiandrosterone | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Glutaredoxin 1 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Glutathione | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Low molecular weight-protein tyrosine phosphatase | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Platelet-derived growth factor | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Vascular smooth muscle cells | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Urata, Yoshishige
× Urata, Yoshishige× Goto, Shinji× Kawakatsu, Miho× Yodoi, Junji× Eto, Masato× Akishita, Masahiro× Kondo, Takahito |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | It is known that dehydroepiandrosterone (DHEA) inhibits a phenotypic switch in vascular smooth muscle cells (VSMC) induced by platelet-derived growth factor (PDGF)-BB. However, the mechanism behind the effect of DHEA on VSMC is not clear. Previously we reported that low molecular weight-protein tyrosine phosphatase (LMW-PTP) dephosphorylates PDGF receptor (PDGFR)-beta via a redox-dependent mechanism involving glutathione (GSH)/glutaredoxin (GRX)1. Here we demonstrate that the redox regulation of PDGFR-beta is involved in the effect of DHEA on VSMC. DHEA suppressed the PDGF-BB-dependent phosphorylation of PDGFR-beta. As expected, DHEA increased the levels of GSH and GRX1, and the GSH/GRX1 system maintained the redox state of LMW-PTP. Down-regulation of the expression of LMW-PTP using siRNA restored the suppression of PDGFR-beta-phosphorylation by DHEA. A promoter analysis of GRX1 and gamma-glutamylcysteine synthetase (gamma-GCS), a rate-limiting enzyme of GSH synthesis, showed that DHEA up-regulated the transcriptional activity at the peroxisome proliferator-activated receptor (PPAR) response element, suggesting PPARalpha plays a role in the induction of GRX1 and gamma-GCS expression by DHEA. In conclusion, the redox regulation of PDGFR-beta is involved in the suppressive effect of DHEA on VSMC proliferation through the up-regulation of GSH/GRX system. | |||||
書誌情報 |
Biochemical and biophysical research communications 巻 396, 号 2, p. 489-494, 発行日 2010-05-28 |
|||||
出版者 | ||||||
出版者 | Elsevier Inc. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0006291X | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 10902104 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00564395 | |||||
PubMed番号 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | PMID | |||||
関連識別子 | 20420805 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.bbrc.2010.04.125 | |||||
権利 | ||||||
権利情報 | Copyright © 2010 Elsevier Inc. All rights reserved. | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Biochemical and biophysical research communications, 396(2), pp.489-494; 2010 |