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Steroid degradation genes in Comamonas testosteroni TA441: Isolation of genes encoding a Delta4(5)-isomerase and 3alpha- and 3beta-dehydrogenases and evidence for a 100kb steroid degradation gene hot spot.
http://hdl.handle.net/10069/23350
http://hdl.handle.net/10069/233507a5f885c-0c31-4022-a340-24eec4ac7c0f
名前 / ファイル | ライセンス | アクション |
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JSBMB_Kudo.pdf (1.3 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2010-07-30 | |||||
タイトル | ||||||
タイトル | Steroid degradation genes in Comamonas testosteroni TA441: Isolation of genes encoding a Delta4(5)-isomerase and 3alpha- and 3beta-dehydrogenases and evidence for a 100kb steroid degradation gene hot spot. | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 3β-Dehydrogenase | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Comamonas testosteroni | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Steroid degradation | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Horinouchi, Masae
× Horinouchi, Masae× Kurita, Tomokazu× Hayashi, Toshiaki× Kudo, Toshiaki |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | In previous studies, we identified two major Comamonas testosteroni TA441 gene clusters involved in steroid degradation. Because most of the genes included in these clusters were revealed to be involved in degradation of basic steroidal structures and a few were suggested to be involved in the degradation of modified steroid compounds, we investigated the spectrum of steroid compounds degradable for TA441 to better identify the genes involved in steroid degradation. TA441 degraded testosterone, progesterone, epiandrosterone, dehydroepiandrosterone, cholic acid, deoxycholic acid, chenodeoxycholic acid, and lithocholic acid. The results suggested TA441 having 3alpha-dehydrogenase and Delta4(5)-isomerase, and 3beta-,17beta-dehydrogenase gene, we isolated these genes, all of which had high homology to the corresponding genes of C. testosteroni ATCC11996. Results of gene-disruption experiments indicated that 3beta,17beta-dehydrogenase is a unique 3beta-dehydrogenase which also acts as a 17beta-dehydrogenase in TA441, and there will be at least one more enzyme with 17beta-dehydrogenating activity. The 3alpha-dehydrogenase and Delta4(5)-isomerase genes were found adjacent in the DNA region between the two main steroid degradation gene clusters together with a number of other genes that may be involved in steroid degradation, suggesting the presence of a steroid degradation gene hot spot over 100kb in size in TA441. | |||||
書誌情報 |
The Journal of steroid biochemistry and molecular biology 巻 122, 号 4, p. 253-263, 発行日 2010-10 |
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出版者 | ||||||
出版者 | Elsevier Ltd | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 09600760 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 18791220 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA1077779X | |||||
PubMed番号 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | PMID | |||||
関連識別子 | 20554032 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.jsbmb.2010.06.002 | |||||
権利 | ||||||
権利情報 | Copyright © 2010 Elsevier Ltd All rights reserved. | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | The Journal of steroid biochemistry and molecular biology, 122(4), pp.253-263; 2010 |