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A novel and specific fluorescence reaction for uracil.
http://hdl.handle.net/10069/23653
http://hdl.handle.net/10069/23653213dfb85-df50-4f2b-a023-8147cf379299
名前 / ファイル | ライセンス | アクション |
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ACA674_234.pdf (1.2 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2010-08-26 | |||||
タイトル | ||||||
タイトル | A novel and specific fluorescence reaction for uracil. | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Benzamidoxime | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Fluorescence reaction | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | High sensitivity | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Pyrimidine metabolism | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Uracil specificity | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Shibata, Takayuki
× Shibata, Takayuki× Kawasaki, Shin-ya× Fujita, Jun-ya× Kabashima, Tsutomu× Kai, Masaaki |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Facile and specific methods to quantify a nucleobase in biological samples are of great importance for diagnosing disorders in nucleic acid metabolism. In the present study, a novel fluorogenic reaction specific for uracil has been developed. The reaction was carried out in an alkaline medium containing benzamidoxime and K(3)[Fe(CN)(6)] which were heated for 2.0 min. Under the optimum reaction conditions, strong fluorescence was produced only from uracil, not from other many biogenic compounds tested such as cytosine, thymine, adenine, guanine, nucleobases, nucleosides, nucleotides, amino acids, saccharides, creatine, creatinine and urea. The sensitivity of this method was compared with a known fluorogenic reaction using phenacylbromide which does not react with uracil but reacts with cytosine, adenine and their analogues. The proposed uracil-specific reaction showed approximately 400-fold higher sensitivity than the phenacylbromide reaction. The lower detection limit of uracil by the present method was 100 pmol mL(-1), and a good linearity of the calibration curve was obtained up to 100 nmol mL(-1) uracil. Due to its high sensitivity and specificity, the quantitative determination of uracil was possible by the proposed fluorimetric method. | |||||
書誌情報 |
Analytica chimica acta 巻 674, 号 2, p. 234-238, 発行日 2010-08-03 |
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出版者 | ||||||
出版者 | Elsevier B.V. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00032670 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 18734324 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00524834 | |||||
PubMed番号 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | PMID | |||||
関連識別子 | 20678635 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.aca.2010.06.028 | |||||
権利 | ||||||
権利情報 | Copyright © 2010 Elsevier B.V. All rights reserved. | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Analytica chimica acta, 674(2), pp.234-238; 2010 |