WEKO3
アイテム
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Immunohistochemical Detection of Human Cellular Yes Gene (C-Yes-1) Messenger Rna by A Non-Radioactive Synthesized Oligodeoxynucleotide Probe
http://hdl.handle.net/10069/23708
http://hdl.handle.net/10069/23708e1bd62ee-01a4-413e-a3d2-ba44c932ae78
名前 / ファイル | ライセンス | アクション |
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AHC22_549.pdf (9.7 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2010-08-31 | |||||
タイトル | ||||||
タイトル | Immunohistochemical Detection of Human Cellular Yes Gene (C-Yes-1) Messenger Rna by A Non-Radioactive Synthesized Oligodeoxynucleotide Probe | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Sugawara, Isamu
× Sugawara, Isamu× Uchino, Kenji× Koji, Takehiko× Sukegawa, Jun× Mori, Shigeo× Nakane, Paul K. |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | A synthetic 39-mer anti-sense strand oligodeoxynucleotide complementary to the region of human cellular yes gene (c-yes-1) mRNA that codes for a polypeptide of 543 amino acids (Mr: 60, 801) and the corresponding sense strand 39-mer oligodeoxynucleotide were chemically synthesized by β-cyanoethyl phospho-ramidite method. The probes thymine-thymine (T-T) dimerized by UV irradiation were used for in situ hybridization studies. Various cells were fixed with ethanol-acetic acid (3:1) at room temperature for 20min and hybridization was carried out in 50% formamide for 36hr at 37°C. The good signal was obtained by utilizing anti-T-T dimer serum (IgG frafction), peroxidase-labeled goat anti-rabbit IgG antibody, and diaminobenzidine-4-HCl and H2O2 as substrates. The anti-sense strand synthetic probe hybridized with cellular RNA of cultured cells with c-yes-1 mRNA, while the sense strand synthetic probe did not. The c-yes-1 mRNA was clearly detected in the cytoplasm of c-yes-1 gene product-expressing cultured cells by in situ hybridization using a T-T dimerized synthetic probe. Synthetic T-T dimerized oligodeoxynucleotides appear to constitute attractive reagents for in situ hybridization studies when supported by appropriate control procedures. | |||||
書誌情報 |
Acta Histochemica et Cytochemica 巻 22, 号 5, p. 549-559, 発行日 1989 |
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出版者 | ||||||
出版者 | The Japan Society of Histochemistry and Cytochemistry | |||||
出版者別言語 | ||||||
日本組織細胞化学会 | ||||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00445991 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13475800 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00508022 | |||||
権利 | ||||||
権利情報 | Copyright ©1989 By the Japan Society of Histochemistry and Cytochemistry | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Acta Histochemica et Cytochemica, 22(5), pp.549-559; 1989 |