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Immunohistochemical and Semiquantitative Immunoblot Analyses of Nm23-Hl and H2 Isoforms in Normal Human Tissues
http://hdl.handle.net/10069/23712
http://hdl.handle.net/10069/237128904e880-c765-4c02-8651-532cf3ad1583
名前 / ファイル | ライセンス | アクション |
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AHC31_411.pdf (11.5 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2010-08-31 | |||||
タイトル | ||||||
タイトル | Immunohistochemical and Semiquantitative Immunoblot Analyses of Nm23-Hl and H2 Isoforms in Normal Human Tissues | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | nm23-H1 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | nm23-H2 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Immunoblotting | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Immunohistochemistry | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | AMeX-fixed tissue | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Tsuchiya, Benio
× Tsuchiya, Benio× Sato, Yuichi× Urano, Takeshi× Baba, Hideo× Shiku, Hiroshi× Kameya, Toru |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The total amount of nm23 protein, the relative ratios of H1 and H2 isoforms (H2/H1) and the localization of these proteins in human normal tissues were studied by a semiquantitative immunoblot technique followed by densitometry and immunohistochemistry with monoclonal antibody against nm23 protein (Pan-242). All tissues contained both isoforms recognized as the 20.5kD H1 protein and 18.5kD H2 protein by immunoblotting. Nm23 protein was abundant in liver, kidney and adrenal gland tissue, and scarce in heart and muscle. H2 levels were always higher than H1, but the isoform ratios (H2/H1) were variable from tissue to tissue. Immunostaining revealed that nm23 protein was predominantly present in cytoplasm and the pattern of staining was homogeneous in parenchymal cells of the liver, pancreas and colonic mucosa and heterogeneous in gastric mucosa and kidney. These results demonstrated that the levels of nm23 protein and the H2/H1 ratios and distribution of isoforms were different in each tissue, and suggests that, when the alterations of nm23 gene expression in tumor tissues are examined, the levels and ratios in non-tumorous tissues surrounding the tumor nest should be considered. | |||||
書誌情報 |
Acta Histochemica et Cytochemica 巻 31, 号 5, p. 411-418, 発行日 1998 |
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出版者 | ||||||
出版者 | The Japan Society of Histochemistry and Cytochemistry | |||||
出版者別言語 | ||||||
日本組織細胞化学会 | ||||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00445991 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13475800 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00508022 | |||||
権利 | ||||||
権利情報 | Copyright ©1998 By the Japan Society of Histochemistry and Cytochemistry | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Acta Histochemica et Cytochemica, 31(5), pp.411-418; 1998 |