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One-step direct reconstitution of biomembranes onto cationic organic polymer bead supports.
http://hdl.handle.net/10069/23993
http://hdl.handle.net/10069/23993ab20c593-0b44-4167-8a33-fda68fc9df8d
名前 / ファイル | ライセンス | アクション |
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JCIS351_96.pdf (3.9 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2010-09-17 | |||||
タイトル | ||||||
タイトル | One-step direct reconstitution of biomembranes onto cationic organic polymer bead supports. | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Biomembrane | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Integral protein | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Ionic polymer | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Reconstitution | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Red blood cell | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Osei-Asante, Samuel
× Osei-Asante, Samuel× Haratake, Mamoru× Fuchigami, Takeshi× Nakayama, Morio |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | In this study, we addressed the straightforward reconstitution of red blood cell (RBC) membranes on the surface of cationic organic polymer beads. The RBC membrane-bead complex was obtained by the incubation of white, unsealed rat RBC ghost membranes with a nonporous quaternary ammonium-type anion-exchange polymer bead with a 350-550 microm diameter. Confocal microscopic observations using a fluorescence membrane probe revealed that the RBC membranes were reconstituted on the outer surface of the bead without any remarkable structural gaps in the membrane. The absence of activity of two peripheral enzymes that latently reside on the cytoplasmic face of the RBC membranes demonstrated that the orientation of the RBC membranes immobilized on the beads was asymmetric as well as that in the native state. The RBC membrane-polymer bead complex was incubated with a primary antibody that is directed against the amino-terminal extracellular domain of the integral protein glycophorin A (GPA). The resulting complex was further incubated with a fluorescent secondary antibody and then subjected to confocal microscopic observations. Fluorescence resulting in the binding of the secondary antibody was found on the surface of the complex, which indicates that the amino-terminal extracellular domain of GPA is exposed to the surface of the complex. In addition, the anion uptake function of the most abundant integral protein anion-exchanger 1 (AE1) immobilized on the polymer beads was inhibited by pretreatment with its specific inhibitor 4,4'-diisothiocyano-2,2'-stilbene disulfonate, as is observed for the intact RBCs. Based on all these results, the RBC membranes were thought to be reconstituted on the ionic polymer beads by our one-pot procedure while maintaining the orientation and functions of the membrane proteins to some extent. | |||||
書誌情報 |
Journal of colloid and interface science 巻 351, 号 1, p. 96-101, 発行日 2010-11-01 |
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出版者 | ||||||
出版者 | Elsevier Inc. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00219797 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 10957103 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00695804 | |||||
PubMed番号 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | PMID | |||||
関連識別子 | 20705302 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.jcis.2010.07.029 | |||||
権利 | ||||||
権利情報 | Copyright © 2010 Elsevier Inc. All rights reserved. | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Journal of colloid and interface science, 351(1), pp.96-101; 2010 |